Successful use of culture and enrichment for the detection of OXA-181-producing Escherichia coli from rectal swab samples falsely categorized as negative by Xpert® Carba-R.
Adult
Bacteriological Techniques
Carrier State
/ diagnosis
Escherichia coli
/ enzymology
Escherichia coli Infections
/ diagnosis
Escherichia coli Proteins
False Negative Reactions
Humans
Male
Molecular Diagnostic Techniques
Reagent Kits, Diagnostic
/ statistics & numerical data
Rectum
/ microbiology
Sensitivity and Specificity
beta-Lactamases
CPE
Rectal swabs
Screening
Journal
Diagnostic microbiology and infectious disease
ISSN: 1879-0070
Titre abrégé: Diagn Microbiol Infect Dis
Pays: United States
ID NLM: 8305899
Informations de publication
Date de publication:
Jan 2020
Jan 2020
Historique:
received:
26
07
2019
revised:
02
10
2019
accepted:
06
10
2019
pubmed:
5
11
2019
medline:
29
4
2020
entrez:
4
11
2019
Statut:
ppublish
Résumé
An OXA-181 producing Escherichia coli isolate that went recurrently undetected directly from rectal swab sample using Xpert® Carba-R, was successfully detected using ertapenem supplemented broth enrichment followed by culture-based method. Our data suggest that implementation of culture-based methods plus enrichment might be crucial for the efficient screening of patients considered to be at "high-risk" of colonization with carbapenemase producers and who are colonized at low level.
Identifiants
pubmed: 31677960
pii: S0732-8893(19)30763-1
doi: 10.1016/j.diagmicrobio.2019.114909
pii:
doi:
Substances chimiques
Escherichia coli Proteins
0
Reagent Kits, Diagnostic
0
beta-Lactamases
EC 3.5.2.6
beta-lactamase OXA-181, E coli
EC 3.5.2.6
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
114909Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.