Reprogramming of DNA methylation at NEUROD2-bound sequences during cortical neuron differentiation.
5-Methylcytosine
/ metabolism
Animals
Base Sequence
Basic Helix-Loop-Helix Transcription Factors
/ metabolism
Cell Differentiation
Cell Line
Cerebral Cortex
/ cytology
DNA Methylation
DNA-Binding Proteins
/ metabolism
Dioxygenases
Enhancer Elements, Genetic
/ genetics
Mice, Inbred C57BL
Mice, Knockout
Neurogenesis
Neurons
/ cytology
Neuropeptides
/ metabolism
Nucleotide Motifs
/ genetics
Oxidation-Reduction
Protein Binding
Proto-Oncogene Proteins
/ metabolism
Journal
Science advances
ISSN: 2375-2548
Titre abrégé: Sci Adv
Pays: United States
ID NLM: 101653440
Informations de publication
Date de publication:
10 2019
10 2019
Historique:
received:
14
02
2019
accepted:
13
09
2019
entrez:
5
11
2019
pubmed:
5
11
2019
medline:
22
5
2020
Statut:
epublish
Résumé
The characteristics of DNA methylation changes that occur during neurogenesis in vivo remain unknown. We used whole-genome bisulfite sequencing to quantitate DNA cytosine modifications in differentiating neurons and their progenitors isolated from mouse brain at the peak of embryonic neurogenesis. Localized DNA hypomethylation was much more common than hypermethylation and often occurred at putative enhancers within genes that were upregulated in neurons and encoded proteins crucial for neuronal differentiation. The hypomethylated regions strongly overlapped with mapped binding sites of the key neuronal transcription factor NEUROD2. The 5-methylcytosine oxidase ten-eleven translocation 2 (TET2) interacted with NEUROD2, and its reaction product 5-hydroxymethylcytosine accumulated at the demethylated regions. NEUROD2-targeted differentially methylated regions retained higher methylation levels in
Identifiants
pubmed: 31681843
doi: 10.1126/sciadv.aax0080
pii: aax0080
pmc: PMC6810389
doi:
Substances chimiques
Basic Helix-Loop-Helix Transcription Factors
0
DNA-Binding Proteins
0
Neurod2 protein, mouse
0
Neuropeptides
0
Proto-Oncogene Proteins
0
5-Methylcytosine
6R795CQT4H
Dioxygenases
EC 1.13.11.-
Tet2 protein, mouse
EC 1.13.11.-
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
eaax0080Subventions
Organisme : NCI NIH HHS
ID : P30 CA033572
Pays : United States
Organisme : NIMH NIH HHS
ID : R01 MH094599
Pays : United States
Informations de copyright
Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).
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