Relationships between the antral follicle count, steroidogenesis, and secretion of follicle-stimulating hormone and anti-Müllerian hormone during follicular growth in cattle.
Animals
Anti-Mullerian Hormone
/ metabolism
Cattle
Cell Count
Cells, Cultured
Estradiol
/ metabolism
Female
Follicle Stimulating Hormone
/ metabolism
Gonadal Steroid Hormones
/ metabolism
Granulosa Cells
/ cytology
Oocytes
/ cytology
Ovarian Follicle
/ cytology
Ovary
/ cytology
Progesterone
/ metabolism
Testosterone
/ metabolism
Anti-Müllerian hormone
Antral follicle count
Follicle stimulating hormone
In vitro growth
Steroidogenesis
Journal
Reproductive biology and endocrinology : RB&E
ISSN: 1477-7827
Titre abrégé: Reprod Biol Endocrinol
Pays: England
ID NLM: 101153627
Informations de publication
Date de publication:
05 Nov 2019
05 Nov 2019
Historique:
received:
11
07
2019
accepted:
15
10
2019
entrez:
7
11
2019
pubmed:
7
11
2019
medline:
21
4
2020
Statut:
epublish
Résumé
The antral follicle count (AFC) in mammalian ovaries positively correlates with female fertility. To clarify the causes of differences in fertility between low and high AFC cows, we investigated follicular growth dynamics and hormone concentrations in plasma, follicular fluid, and in vitro growth (IVG) media at different stages of follicular growth. Seven cows were divided into high AFC (n = 4, > 30 follicles) and low AFC (n = 3, < 30 follicles) groups based on the peak AFC detected by ultrasonography. These cows were subjected to estrous synchronization, daily ovarian ultrasonography, and blood collection. Their follicular fluid was collected from dominant follicles at different stages (selection, luteal, and ovulatory phases). In another experiment, we cultured oocyte-cumulus-granulosa cell complexes collected from early antral follicles (< 1 mm) for 12 days. Estradiol-17β (E The numbers of small (< 4 mm) and intermediate (4-8 mm) follicles were larger in the high AFC group than in the low AFC group (P < 0.05). The number of intermediate follicles was stable in the low AFC group, indicating consistent development. However, the number of these follicles fluctuated in the high AFC group. Plasma FSH concentrations were higher, whereas E The weaker response to FSH of granulosa cells caused low E
Sections du résumé
BACKGROUND
BACKGROUND
The antral follicle count (AFC) in mammalian ovaries positively correlates with female fertility. To clarify the causes of differences in fertility between low and high AFC cows, we investigated follicular growth dynamics and hormone concentrations in plasma, follicular fluid, and in vitro growth (IVG) media at different stages of follicular growth.
METHODS
METHODS
Seven cows were divided into high AFC (n = 4, > 30 follicles) and low AFC (n = 3, < 30 follicles) groups based on the peak AFC detected by ultrasonography. These cows were subjected to estrous synchronization, daily ovarian ultrasonography, and blood collection. Their follicular fluid was collected from dominant follicles at different stages (selection, luteal, and ovulatory phases). In another experiment, we cultured oocyte-cumulus-granulosa cell complexes collected from early antral follicles (< 1 mm) for 12 days. Estradiol-17β (E
RESULTS
RESULTS
The numbers of small (< 4 mm) and intermediate (4-8 mm) follicles were larger in the high AFC group than in the low AFC group (P < 0.05). The number of intermediate follicles was stable in the low AFC group, indicating consistent development. However, the number of these follicles fluctuated in the high AFC group. Plasma FSH concentrations were higher, whereas E
CONCLUSIONS
CONCLUSIONS
The weaker response to FSH of granulosa cells caused low E
Identifiants
pubmed: 31690325
doi: 10.1186/s12958-019-0534-3
pii: 10.1186/s12958-019-0534-3
pmc: PMC6833202
doi:
Substances chimiques
Gonadal Steroid Hormones
0
Testosterone
3XMK78S47O
Progesterone
4G7DS2Q64Y
Estradiol
4TI98Z838E
Anti-Mullerian Hormone
80497-65-0
Follicle Stimulating Hormone
9002-68-0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
88Subventions
Organisme : Japan Society for the Promotion of Science
ID : JP16K08043
Organisme : Japan Society for the Promotion of Science
ID : JP19H03117
Organisme : Japan Society for the Promotion of Science
ID : JP18J13899
Références
Domest Anim Endocrinol. 2016 Oct;57:85-99
pubmed: 27565235
Biol Reprod. 2005 Jul;73(1):54-62
pubmed: 15744026
Reproduction. 2012 May;143(5):673-82
pubmed: 22367589
J Clin Endocrinol Metab. 2011 Mar;96(3):746-54
pubmed: 21159842
PLoS One. 2013 Apr 23;8(4):e61095
pubmed: 23637787
J Hum Reprod Sci. 2013 Jan;6(1):27-31
pubmed: 23869147
J Reprod Dev. 2006 Feb;52(1):129-35
pubmed: 16293945
Fertil Steril. 2011 Nov;96(5):1246-51.e1
pubmed: 21917251
Biol Reprod. 2004 Jan;70(1):83-91
pubmed: 12954724
Biol Reprod. 2006 Aug;75(2):217-25
pubmed: 16641147
Hum Reprod Update. 2002 Mar-Apr;8(2):141-54
pubmed: 12099629
Reprod Biomed Online. 2015 Oct;31(4):486-96
pubmed: 26283017
Theriogenology. 1994 Nov 1;42(6):895-907
pubmed: 16727595
Eur J Obstet Gynecol Reprod Biol. 2013 Jun;168(2):173-7
pubmed: 23485092
Theriogenology. 1997 Oct 1;48(5):743-55
pubmed: 16728168
J Dairy Sci. 2012 May;95(5):2355-61
pubmed: 22541464
Endocrinology. 2001 Nov;142(11):4891-9
pubmed: 11606457
Reproduction. 2010 Nov;140(5):713-20
pubmed: 20699380
Biol Reprod. 2009 May;80(5):954-64
pubmed: 19164170
Biol Reprod. 2008 Dec;79(6):1219-25
pubmed: 18768912
Mol Hum Reprod. 2017 May 1;23(5):282-291
pubmed: 28333275
Hum Reprod Update. 2006 Nov-Dec;12(6):685-718
pubmed: 16891297
Reproduction. 2017 Jul;154(1):R1-R11
pubmed: 28356501
Mol Reprod Dev. 2002 Feb;61(2):234-48
pubmed: 11803560
Reprod Fertil Dev. 2012;24(2):327-36
pubmed: 22281079
Biol Reprod. 2008 Aug;79(2):387-96
pubmed: 18448844
Fertil Steril. 2013 Aug;100(2):585-92.e1
pubmed: 23663993
Anim Reprod Sci. 2015 Dec;163:172-8
pubmed: 26588889
J Reprod Dev. 2015;61(1):61-6
pubmed: 25482110
Clin Endocrinol (Oxf). 2006 Jun;64(6):603-10
pubmed: 16712660
J Reprod Dev. 2018 Dec 14;64(6):503-510
pubmed: 30135328
Endocrinology. 1999 Dec;140(12):5789-96
pubmed: 10579345
Fertil Steril. 2009 Mar;91(3):705-14
pubmed: 18321493
Biol Reprod. 2000 Aug;63(2):383-9
pubmed: 10906041
Fertil Steril. 2013 May;99(6):1644-53
pubmed: 23394782
Reprod Biol Endocrinol. 2013 Dec 17;11:115
pubmed: 24341292
Arch Gynecol Obstet. 2014 Dec;290(6):1249-53
pubmed: 25001569
Fertil Steril. 2008 May;89(5 Suppl):1364-70
pubmed: 17517397
J Clin Endocrinol Metab. 2013 Mar;98(3):1107-14
pubmed: 23408576
J Dairy Sci. 2000 Jan;83(1):62-9
pubmed: 10659965
Hum Reprod. 2011 Jul;26(7):1768-74
pubmed: 21558332
Reproduction. 2013 Jan 08;145(1):85-96
pubmed: 23129663
Biol Reprod. 1997 May;56(5):1158-68
pubmed: 9160714
Theriogenology. 2017 Jul 15;97:113-123
pubmed: 28583594
Hum Reprod. 2007 Jun;22(6):1687-95
pubmed: 17468258
Reprod Fertil Dev. 2010;22(3):550-7
pubmed: 20188028
Front Neuroendocrinol. 1996 Oct;17(4):476-509
pubmed: 8905350
Endocrinology. 2012 Sep;153(9):4533-43
pubmed: 22778215
Reprod Biol Endocrinol. 2016 Jan 15;14:3
pubmed: 26769429
Hum Reprod Update. 2012 Jan-Feb;18(1):73-91
pubmed: 22068695
Biomed Res. 2016;37(1):63-71
pubmed: 26912141
J Hum Reprod Sci. 2012 Sep;5(3):274-8
pubmed: 23531919
Reprod Fertil Dev. 2011;23(1):1-14
pubmed: 21366975
J Anim Sci. 1966 Aug;25(3):800-5
pubmed: 6007918
Biol Reprod. 2002 Jul;67(1):38-45
pubmed: 12079997
Fertil Steril. 2012 Jan;97(1):238-43
pubmed: 22130324
Reprod Fertil Dev. 2016 Mar;28(3):349-56
pubmed: 25029376
J Anim Sci. 2019 Apr 3;97(4):1446-1455
pubmed: 30668706
Hum Reprod Update. 2011 Jan-Feb;17(1):46-54
pubmed: 20667894
Biol Reprod. 2009 Jan;80(1):50-9
pubmed: 18784351