Impact of humanised isolation and culture conditions on stemness and osteogenic potential of bone marrow derived mesenchymal stromal cells.

Animals Bone Marrow Cells / cytology Cell Culture Techniques Cell Differentiation Cell Separation Heterografts Humans Mesenchymal Stem Cell Transplantation Mesenchymal Stem Cells / cytology Mice Mice, Inbred NOD Mice, SCID Osteogenesis

Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
05 11 2019
Historique:
received: 07 06 2019
accepted: 13 10 2019
entrez: 7 11 2019
pubmed: 7 11 2019
medline: 28 10 2020
Statut: epublish

Résumé

Therapeutic potential of human bone marrow stromal/stem cells (hBMSC) must be developed using well defined xenogenic-free conditions. hBMSC were isolated from healthy donors (n = 3) using different isolation and expansion methods. Donor I was isolated and expanded by either bone marrow directly seeded and cells expanded in 10% AB human serum (AB) +5 ng/ml fibroblast growth factor-2 (FGF2) [Direct(AB + FGF

Identifiants

DOI: 10.1038/s41598-019-52442-9 PMID: 31690774 PMC: PMC6831606
pubmed: 31690774
doi: 10.1038/s41598-019-52442-9
pii: 10.1038/s41598-019-52442-9
pmc: PMC6831606
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

16031

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Auteurs

Salwa Suliman (S)

Department of Clinical Dentistry, Centre for Clinical Dental Research, University of Bergen, Bergen, Norway. salwa.suliman@uib.no.

Hassan R W Ali (HRW)

Department of Clinical Dentistry, Centre for Clinical Dental Research, University of Bergen, Bergen, Norway.

Tommy A Karlsen (TA)

Norwegian Center for Stem Cell Research, Department of Immunology, Oslo University Hospital Rikshospitalet, Oslo, Norway.

Jerome Amiaud (J)

INSERM, UMR 1238, PHY-OS, Laboratory of Bone Sarcomas and Remodeling of Calcified Tissues, Faculty of Medicine, University of Nantes, Nantes, France.

Samih Mohamed-Ahmed (S)

Department of Clinical Dentistry, Centre for Clinical Dental Research, University of Bergen, Bergen, Norway.

Pierre Layrolle (P)

INSERM, UMR 1238, PHY-OS, Laboratory of Bone Sarcomas and Remodeling of Calcified Tissues, Faculty of Medicine, University of Nantes, Nantes, France.

Daniela E Costea (DE)

Gade Laboratory for Pathology, Department of Clinical Medicine, University of Bergen, Bergen, Norway.
Department of Pathology, Haukeland University Hospital, Bergen, Norway.
Centre for Cancer Biomarkers, University of Bergen, Bergen, Norway.

Jan E Brinchmann (JE)

Norwegian Center for Stem Cell Research, Department of Immunology, Oslo University Hospital Rikshospitalet, Oslo, Norway.
Department of Molecular Medicine, Faculty of Medicine, University of Oslo, Oslo, Norway.

Kamal Mustafa (K)

Department of Clinical Dentistry, Centre for Clinical Dental Research, University of Bergen, Bergen, Norway. kamal.mustafa@uib.no.

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Classifications MeSH

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