Early Growth Response Genes Increases Rapidly After Mechanical Overloading and Unloading in Tendon Constructs.
EGR1
EGR2
mechanical loading
tenocytes
tension release
Journal
Journal of orthopaedic research : official publication of the Orthopaedic Research Society
ISSN: 1554-527X
Titre abrégé: J Orthop Res
Pays: United States
ID NLM: 8404726
Informations de publication
Date de publication:
01 2020
01 2020
Historique:
received:
27
03
2019
accepted:
03
11
2019
pubmed:
7
11
2019
medline:
14
2
2020
entrez:
7
11
2019
Statut:
ppublish
Résumé
Tendon cells exist in a dense extracellular matrix and mechanical loading is important for the strength development of this matrix. We therefore use a three-dimensional (3D) culture system for tendon formation in vitro. The objectives of this study were to elucidate the temporal expression of tendon-related genes during the formation of artificial tendons in vitro and to investigate if early growth response-1 (EGR1), EGR2, FOS, and cyclooxygenase-1 and -2 (PTGS1 and PTGS2) are sensitive to mechanical loading. First, we studied messenger RNA (mRNA) levels of several tendon-related genes during formation of tendon constructs. Second, we studied the mRNA levels of, for example, EGR1 and EGR2 after different degrees of loading; dynamic physiologic-range loading (2.5% strain), dynamic overloading (approximately 10% strain), or tension release. The gene expression for tendon-related genes (i.e., EGR2, MKX, TNMD, COL3A1) increased with time after seeding into this 3D model. EGR1, EGR2, FOS, PTGS1, and PTGS2 did not respond to physiologic-range loading. But overloading (and tension release) lead to elevated levels of EGR1 and EGR2 (p ≤ 0.006). FOS and PTGS2 were increased after overloading (both p < 0.007) but not after tension release (p = 0.06 and 0.08). In conclusion, the expression of tendon-related genes increases during the formation of artificial tendons in vitro, including EGR2. Furthermore, the gene expression of EGR1 and EGR2 in human tendon cells appear to be sensitive to overloading and unloading but did not respond to the single episode of physiologic-range loading. These findings could be helpful for the understanding of tendon tensional homeostasis. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 38:173-181, 2020.
Substances chimiques
EGR1 protein, human
0
EGR2 protein, human
0
Early Growth Response Protein 1
0
Early Growth Response Protein 2
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
173-181Subventions
Organisme : Lundbeck Foundation
ID : R198-2015-207
Pays : International
Organisme : Nordea Foundation
ID : NF-007IOC
Pays : International
Organisme : IOC Sports Medicine Copenhagen
Pays : International
Organisme : Danish Medical Research Council
ID : 0602-02960B
Pays : International
Organisme : Swedish Society for Medical Research
Pays : International
Organisme : Lions Research Foundation
Pays : International
Organisme : Magnus Bergvall Foundation
ID : 2015-01169
Pays : International
Organisme : Magnus Bergvall Foundation
ID : 2016-01811
Pays : International
Organisme : Swedish Research Council for Sport Science
ID : D2017-0021
Pays : International
Organisme : Swedish Research Council for Sport Science
ID : P2017-0109
Pays : International
Organisme : Swedish Fund for Research without Animal Experiments
Pays : International
Informations de copyright
© 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.
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