An ultra high-throughput method for single-cell joint analysis of open chromatin and transcriptome.
Journal
Nature structural & molecular biology
ISSN: 1545-9985
Titre abrégé: Nat Struct Mol Biol
Pays: United States
ID NLM: 101186374
Informations de publication
Date de publication:
11 2019
11 2019
Historique:
received:
11
06
2019
accepted:
30
09
2019
pubmed:
7
11
2019
medline:
20
2
2020
entrez:
8
11
2019
Statut:
ppublish
Résumé
Simultaneous profiling of transcriptome and chromatin accessibility within single cells is a powerful approach to dissect gene regulatory programs in complex tissues. However, current tools are limited by modest throughput. We now describe an ultra high-throughput method, Paired-seq, for parallel analysis of transcriptome and accessible chromatin in millions of single cells. We demonstrate the utility of Paired-seq for analyzing the dynamic and cell-type-specific gene regulatory programs in complex tissues by applying it to mouse adult cerebral cortex and fetal forebrain. The joint profiles of a large number of single cells allowed us to deconvolute the transcriptome and open chromatin landscapes in the major cell types within these brain tissues, infer putative target genes of candidate enhancers, and reconstruct the trajectory of cellular lineages within the developing forebrain.
Identifiants
pubmed: 31695190
doi: 10.1038/s41594-019-0323-x
pii: 10.1038/s41594-019-0323-x
pmc: PMC7231560
mid: NIHMS1561987
doi:
Substances chimiques
Chromatin
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1063-1070Subventions
Organisme : NIMH NIH HHS
ID : U19 MH114831
Pays : United States
Organisme : NHGRI NIH HHS
ID : U54 HG006997
Pays : United States
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