Comparison of the metabolism of 10 cosmetics-relevant chemicals in EpiSkin™ S9 subcellular fractions and in vitro human skin explants.
Acetophenones
/ metabolism
Aniline Compounds
/ metabolism
Animals
Benzaldehydes
/ metabolism
Benzylamines
/ metabolism
Caffeine
/ metabolism
Cells, Cultured
/ drug effects
Cosmetics
/ metabolism
Humans
Parabens
/ metabolism
Pentanoic Acids
/ metabolism
Propanols
/ metabolism
Resorcinols
/ metabolism
Skin
/ drug effects
Skin Irritancy Tests
/ methods
EpiSkin S9 fractions
comparison
human skin explants
metabolism
parent chemical depletion
Journal
Journal of applied toxicology : JAT
ISSN: 1099-1263
Titre abrégé: J Appl Toxicol
Pays: England
ID NLM: 8109495
Informations de publication
Date de publication:
02 2020
02 2020
Historique:
received:
01
02
2019
revised:
13
08
2019
accepted:
27
08
2019
pubmed:
9
11
2019
medline:
6
7
2021
entrez:
9
11
2019
Statut:
ppublish
Résumé
An understanding of the bioavailability of topically applied cosmetics ingredients is key to predicting their local skin and systemic toxicity and making a safety assessment. We investigated whether short-term incubations with S9 from the reconstructed epidermal skin model, EpiSkin™, would give an indication of the rate of chemical metabolism and produce similar metabolites to those formed in incubations with human skin explants. Both have advantages: EpiSkin™ S9 is a higher-throughput assay, while the human skin explant model represents a longer incubation duration (24 hours) model integrating cutaneous distribution with metabolite formation. Here, we compared the metabolism of 10 chemicals (caffeine, vanillin, cinnamyl alcohol, propylparaben, 4-amino-3-nitrophenol, resorcinol, 4-chloroaniline, 2-amino-3-methyl-3H-imidazo[4,5-F]quinoline and 2-acetyl aminofluorene) in both models. Both models were shown to have functional Phase 1 and 2 enzymes, including cytochrome P450 activities. There was a good concordance between the models with respect to the level of metabolism (stable vs. slowly vs. extensively metabolized chemicals) and major early metabolites produced for eight chemicals. Discordant results for two chemicals were attributed to a lack of the appropriate cofactor (NADP
Substances chimiques
Acetophenones
0
Aniline Compounds
0
Benzaldehydes
0
Benzylamines
0
Cosmetics
0
Parabens
0
Pentanoic Acids
0
Propanols
0
Resorcinols
0
Caffeine
3G6A5W338E
Types de publication
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
313-326Informations de copyright
© 2019 John Wiley & Sons, Ltd.
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