The Biogenesis of SRP RNA Is Modulated by an RNA Folding Intermediate Attained during Transcription.

Escherichia coli / genetics Protein Binding / genetics RNA / genetics RNA Folding / genetics Ribosomes / genetics Signal Recognition Particle / genetics Transcription, Genetic / genetics
RNA maturation SRP SRP RNA co-transcriptional RNA-folding trajectory folding intermediates optical tweezers signal recognition particle single-molecule analysis transcription

Journal

Molecular cell
ISSN: 1097-4164
Titre abrégé: Mol Cell
Pays: United States
ID NLM: 9802571

Informations de publication

Date de publication:
16 01 2020
Historique:
received: 18 08 2018
revised: 29 08 2019
accepted: 04 10 2019
pubmed: 11 11 2019
medline: 1 4 2020
entrez: 11 11 2019
Statut: ppublish

Résumé

The signal recognition particle (SRP), responsible for co-translational protein targeting and delivery to cellular membranes, depends on the native long-hairpin fold of its RNA to confer functionality. Since RNA initiates folding during its synthesis, we used high-resolution optical tweezers to follow in real time the co-transcriptional folding of SRP RNA. Surprisingly, SRP RNA folding is robust to transcription rate changes and the presence or absence of its 5'-precursor sequence. The folding pathway also reveals the obligatory attainment of a non-native hairpin intermediate (H1) that eventually rearranges into the native fold. Furthermore, H1 provides a structural platform alternative to the native fold for RNase P to bind and mature SRP RNA co-transcriptionally. Delays in attaining the final native fold are detrimental to the cell, altogether showing that a co-transcriptional folding pathway underpins the proper biogenesis of function-essential SRP RNA.

Identifiants

DOI: 10.1016/j.molcel.2019.10.006 PMID: 31706702
pubmed: 31706702
pii: S1097-2765(19)30766-X
doi: 10.1016/j.molcel.2019.10.006
pii:
doi:

Substances chimiques

Signal Recognition Particle 0
RNA 63231-63-0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

241-250.e8

Informations de copyright

Published by Elsevier Inc.

Auteurs

Shingo Fukuda (S)

Institute for Quantitative Biosciences-QB3, University of California, Berkeley, Berkeley, CA, USA; Okazaki Institute for Integrative Bioscience and Institute for Molecular Science, National Institutes of Natural Science, Tokyo, Japan; Nano Life Science Institute (WPI-NanoLSI), Kanazawa University, Kanazawa 920-1192, Japan. Electronic address: shingof0617@gmail.com.

Shannon Yan (S)

Institute for Quantitative Biosciences-QB3, University of California, Berkeley, Berkeley, CA, USA; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA. Electronic address: shannonyan@berkeley.edu.

Yusuke Komi (Y)

Institute for Quantitative Biosciences-QB3, University of California, Berkeley, Berkeley, CA, USA; Laboratory for Protein Conformation Diseases, RIKEN Center for Brain Science, Wako, Saitama, Japan.

Mingxuan Sun (M)

Institute for Quantitative Biosciences-QB3, University of California, Berkeley, Berkeley, CA, USA; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA.

Ronen Gabizon (R)

Institute for Quantitative Biosciences-QB3, University of California, Berkeley, Berkeley, CA, USA.

Carlos Bustamante (C)

Institute for Quantitative Biosciences-QB3, University of California, Berkeley, Berkeley, CA, USA; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA; Jason L. Choy Laboratory of Single-Molecule Biophysics, University of California, Berkeley, Berkeley, CA, USA; Biophysics Graduate Group, University of California, Berkeley, Berkeley, CA, USA; Department of Chemistry, University of California, Berkeley, Berkeley, CA, USA; Department of Physics, University of California, Berkeley, Berkeley, CA, USA; Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA, USA; Kavli Energy Nanoscience Institute, University of California, Berkeley, Berkeley, CA, USA. Electronic address: carlosb@berkeley.edu.

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Classifications MeSH

questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Enterobacteriaceae › Escherichia › Escherichia coli › The Biogenesis of SRP RNA Is Modulated by an...
questionsmedicales.fr › Métabolisme › Liaison aux protéines › The Biogenesis of SRP RNA Is Modulated by an...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › The Biogenesis of SRP RNA Is Modulated by an...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Conformation d'acide nucléique › Pliage de l'ARN › The Biogenesis of SRP RNA Is Modulated by an...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Organites › Ribosomes › The Biogenesis of SRP RNA Is Modulated by an...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Nucléoprotéines › Protéines de liaison à l'ARN › Ribonucléoprotéines › Petites ribonucléoprotéines cytoplasmiques › Particule de reconnaissance du signal › The Biogenesis of SRP RNA Is Modulated by an...
questionsmedicales.fr › Phénomènes génétiques › Expression des gènes › Transcription génétique › The Biogenesis of SRP RNA Is Modulated by an...