Niacin protects against abdominal aortic aneurysm formation via GPR109A independent mechanisms: role of NAD+/nicotinamide.


Journal

Cardiovascular research
ISSN: 1755-3245
Titre abrégé: Cardiovasc Res
Pays: England
ID NLM: 0077427

Informations de publication

Date de publication:
01 12 2020
Historique:
received: 22 05 2019
revised: 23 10 2019
accepted: 07 11 2019
pubmed: 12 11 2019
medline: 24 8 2021
entrez: 12 11 2019
Statut: ppublish

Résumé

Chronic adventitial and medial infiltration of immune cells play an important role in the pathogenesis of abdominal aortic aneurysms (AAAs). Nicotinic acid (niacin) was shown to inhibit atherosclerosis by activating the anti-inflammatory G protein-coupled receptor GPR109A [also known as hydroxycarboxylic acid receptor 2 (HCA2)] expressed on immune cells, blunting immune activation and adventitial inflammatory cell infiltration. Here, we investigated the role of niacin and GPR109A in regulating AAA formation. Mice were supplemented with niacin or nicotinamide, and AAA was induced by angiotensin II (AngII) infusion or calcium chloride (CaCl2) application. Niacin markedly reduced AAA formation in both AngII and CaCl2 models, diminishing adventitial immune cell infiltration, concomitant inflammatory responses, and matrix degradation. Unexpectedly, GPR109A gene deletion did not abrogate the protective effects of niacin against AAA formation, suggesting GPR109A-independent mechanisms. Interestingly, nicotinamide, which does not activate GPR109A, also inhibited AAA formation and phenocopied the effects of niacin. Mechanistically, both niacin and nicotinamide supplementation increased nicotinamide adenine dinucleotide (NAD+) levels and NAD+-dependent Sirt1 activity, which were reduced in AAA tissues. Furthermore, pharmacological inhibition of Sirt1 abrogated the protective effect of nicotinamide against AAA formation. Niacin protects against AAA formation independent of GPR109A, most likely by serving as an NAD+ precursor. Supplementation of NAD+ using nicotinamide-related biomolecules may represent an effective and well-tolerated approach to preventing or treating AAA.

Identifiants

pubmed: 31710686
pii: 5618721
doi: 10.1093/cvr/cvz303
pmc: PMC7695356
doi:

Substances chimiques

Hcar2 protein, mouse 0
Receptors, G-Protein-Coupled 0
Receptors, LDL 0
NAD 0U46U6E8UK
Angiotensin II 11128-99-7
Niacinamide 25X51I8RD4
Niacin 2679MF687A
Sirt1 protein, mouse EC 3.5.1.-
Sirtuin 1 EC 3.5.1.-
Calcium Chloride M4I0D6VV5M

Types de publication

Journal Article Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

2226-2238

Subventions

Organisme : NEI NIH HHS
ID : R01 EY029318
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL126949
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL142097
Pays : United States

Commentaires et corrections

Type : CommentIn

Informations de copyright

© The Author(s) 2019. Published by Oxford University Press on behalf of the European Society of Cardiology.

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Auteurs

Tetsuo Horimatsu (T)

Department of Medicine, Medical College of Georgia, Augusta University, Augusta, GA, USA.
Department of Vascular Biology Center, Medical College of Georgia, Augusta University, 1459 Laney Walker Blvd, Augusta, GA 30912, USA.

Andra L Blomkalns (AL)

Department of Emergency Medicine, University of Cincinnati College of Medicine, Cincinnati, OH, USA.

Mourad Ogbi (M)

Department of Medicine, Medical College of Georgia, Augusta University, Augusta, GA, USA.
Department of Vascular Biology Center, Medical College of Georgia, Augusta University, 1459 Laney Walker Blvd, Augusta, GA 30912, USA.

Mary Moses (M)

Department of Medicine, Medical College of Georgia, Augusta University, Augusta, GA, USA.
Department of Vascular Biology Center, Medical College of Georgia, Augusta University, 1459 Laney Walker Blvd, Augusta, GA 30912, USA.

David Kim (D)

Department of Medicine, Medical College of Georgia, Augusta University, Augusta, GA, USA.
Department of Vascular Biology Center, Medical College of Georgia, Augusta University, 1459 Laney Walker Blvd, Augusta, GA 30912, USA.

Sagar Patel (S)

Department of Medicine, Medical College of Georgia, Augusta University, Augusta, GA, USA.
Department of Vascular Biology Center, Medical College of Georgia, Augusta University, 1459 Laney Walker Blvd, Augusta, GA 30912, USA.

Nicole Gilreath (N)

Department of Medicine, Medical College of Georgia, Augusta University, Augusta, GA, USA.
Department of Vascular Biology Center, Medical College of Georgia, Augusta University, 1459 Laney Walker Blvd, Augusta, GA 30912, USA.

Lauren Reid (L)

Department of Medicine, Medical College of Georgia, Augusta University, Augusta, GA, USA.
Department of Vascular Biology Center, Medical College of Georgia, Augusta University, 1459 Laney Walker Blvd, Augusta, GA 30912, USA.

Tyler W Benson (TW)

Department of Medicine, Medical College of Georgia, Augusta University, Augusta, GA, USA.
Department of Vascular Biology Center, Medical College of Georgia, Augusta University, 1459 Laney Walker Blvd, Augusta, GA 30912, USA.

Jonathan Pye (J)

Department of Medicine, Medical College of Georgia, Augusta University, Augusta, GA, USA.
Department of Vascular Biology Center, Medical College of Georgia, Augusta University, 1459 Laney Walker Blvd, Augusta, GA 30912, USA.

Samah Ahmadieh (S)

Department of Medicine, Medical College of Georgia, Augusta University, Augusta, GA, USA.
Department of Vascular Biology Center, Medical College of Georgia, Augusta University, 1459 Laney Walker Blvd, Augusta, GA 30912, USA.

Allie Thompson (A)

Department of Emergency Medicine, University of Cincinnati College of Medicine, Cincinnati, OH, USA.

Nathan Robbins (N)

Department of Emergency Medicine, University of Cincinnati College of Medicine, Cincinnati, OH, USA.

Adrien Mann (A)

Department of Emergency Medicine, University of Cincinnati College of Medicine, Cincinnati, OH, USA.

Ashlee Edgell (A)

Department of Emergency Medicine, University of Cincinnati College of Medicine, Cincinnati, OH, USA.

Stephanie Benjamin (S)

Department of Emergency Medicine, University of Cincinnati College of Medicine, Cincinnati, OH, USA.

Brian K Stansfield (BK)

Department of Vascular Biology Center, Medical College of Georgia, Augusta University, 1459 Laney Walker Blvd, Augusta, GA 30912, USA.
Department of Pediatrics, Medical College of Georgia, Augusta University, Augusta, GA, USA.

Yuqing Huo (Y)

Department of Vascular Biology Center, Medical College of Georgia, Augusta University, 1459 Laney Walker Blvd, Augusta, GA 30912, USA.
Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta University, Augusta, GA, USA.

David J Fulton (DJ)

Department of Vascular Biology Center, Medical College of Georgia, Augusta University, 1459 Laney Walker Blvd, Augusta, GA 30912, USA.
Department of Pharmacology and Toxicology, Medical College of Georgia, Augusta University, Augusta, GA, USA.

Gautam Agarwal (G)

Department of Surgery, Medical College of Georgia, Augusta University, Augusta, GA, USA.

Nagendra Singh (N)

Biochemistry and Molecular Biology, Medical College of Georgia, Augusta University, Augusta, GA, USA.

Stefan Offermanns (S)

Department of Pharmacology, Max Planck Institute for Heart and Lung Research, Ludwigstraße, Bad Nauheim, Germany.

Neal L Weintraub (NL)

Department of Medicine, Medical College of Georgia, Augusta University, Augusta, GA, USA.
Department of Vascular Biology Center, Medical College of Georgia, Augusta University, 1459 Laney Walker Blvd, Augusta, GA 30912, USA.

Ha Won Kim (HW)

Department of Medicine, Medical College of Georgia, Augusta University, Augusta, GA, USA.
Department of Vascular Biology Center, Medical College of Georgia, Augusta University, 1459 Laney Walker Blvd, Augusta, GA 30912, USA.

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Classifications MeSH