Marker-less deletion of cctA gene of Clostridium chauvoei.
Animal Diseases
/ microbiology
Animals
Animals, Domestic
Anti-Bacterial Agents
/ pharmacology
Antibiotic Prophylaxis
Bacterial Proteins
/ genetics
CRISPR-Cas Systems
Clostridium Infections
/ veterinary
Clostridium chauvoei
/ drug effects
Gene Deletion
Gene Editing
Hemolysin Proteins
/ genetics
Hemolysis
Mutation
CRISPR-Cas9
Clostridium chauvoei
Genome editing
cctA
Journal
Anaerobe
ISSN: 1095-8274
Titre abrégé: Anaerobe
Pays: England
ID NLM: 9505216
Informations de publication
Date de publication:
Feb 2020
Feb 2020
Historique:
received:
05
12
2018
revised:
18
10
2019
accepted:
07
11
2019
pubmed:
13
11
2019
medline:
30
10
2020
entrez:
13
11
2019
Statut:
ppublish
Résumé
Clostridium chauvoei causes blackleg disease in domestic animals, especially cattle and sheep. The pathogen produces several toxins including CctA - a hemolysin and protective antigen. Molecular pathogenesis of the disease is poorly understood, possibly due to lack of genetic manipulation tools for C. chauvoei. In the present study, we report the marker-less deletion of cctA gene using the CRISPR-Cas9 system. The C. chauvoei cctA deletion mutant had negligible hemolytic and significantly reduced cytotoxic activities. To the best of our knowledge, this is the first report of genetic manipulation of C. chauvoei. The method we used in this study can be applied for genetic manipulation of C. chauvoei to better understand the pathogenesis and genetics of the pathogen.
Identifiants
pubmed: 31711886
pii: S1075-9964(19)30188-X
doi: 10.1016/j.anaerobe.2019.102116
pii:
doi:
Substances chimiques
Anti-Bacterial Agents
0
Bacterial Proteins
0
Hemolysin Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
102116Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest Authors declare no conflicts of interest.