The application of autophagy to thrombus age estimation in murine deep vein thrombosis model.
autophagy
forensic pathology
immunohistochemistry
light chain 3
p62
thrombus age determination
Journal
International journal of legal medicine
ISSN: 1437-1596
Titre abrégé: Int J Legal Med
Pays: Germany
ID NLM: 9101456
Informations de publication
Date de publication:
May 2020
May 2020
Historique:
received:
16
04
2019
accepted:
07
10
2019
pubmed:
13
11
2019
medline:
4
2
2021
entrez:
13
11
2019
Statut:
ppublish
Résumé
We immunohistochemically examined the intrathrombotic dynamics of autophagy during thrombogenesis using murine deep vein thrombosis (DVT) models. To perform the immunohistochemical analyses, we used anti-LC3 antibody and anti-p62 antibody for detecting the intrathrombotic autophagic functions. We estimated dynamics of the intrathrombotic autophagy as LC3
Identifiants
pubmed: 31713680
doi: 10.1007/s00414-019-02168-0
pii: 10.1007/s00414-019-02168-0
doi:
Substances chimiques
Map1lc3b protein, mouse
0
Microtubule-Associated Proteins
0
Sequestosome-1 Protein
0
Sqstm1 protein, mouse
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1061-1066Subventions
Organisme : Ministry of Education, Culture, Sports, Science, and Technology of the Japanese Government
ID : Grants-in-Aid for Scientific Research (A)
Organisme : Ministry of Education, Culture, Sports, Science, and Technology of the Japanese Government
ID : Grants-in-Aid for Scientific Research (C)
Références
Kimura A, Ishida Y, Wada T, Hisaoka T, Morikawa Y, Sugaya T, Mukaida N, Kondo T (2010) The absence of interleukin-6 enhanced arsenite-induced renal injury by promoting autophagy of tubular epithelial cells with aberrant extracellular signal-regulated kinase activation. Am J Pathol 176:40–50. https://doi.org/10.2353/ajpath.2010.090146
doi: 10.2353/ajpath.2010.090146
pubmed: 20008137
pmcid: 2797868
Kimura A, Ishida Y, Nosaka M, Shiraki M, Hama M, Kawaguchi T, Kuninaka Y, Shimada E, Yamamoto H, Takayasu T, Kondo T (2015) Autophagy in skin wounds: a novel marker for vital reactions. Int J Legal Med 129:537–541. https://doi.org/10.1007/s00414-015-1168-4
doi: 10.1007/s00414-015-1168-4
pubmed: 25753383
Komatsu M, Waguri S, Chiba T, Murata S, Iwata J, Tanida I, Ueno T, Koike M, Uchiyama Y, Kominami E, Tanaka K (2006) Loss of autophagy in the central nervous system causes neurodegeneration in mice. Nature 441:880–884. https://doi.org/10.1038/nature04723
doi: 10.1038/nature04723
pubmed: 16625205
Tang DY, Ellis RA, Lovat PE (2016) Prognostic impact of autophagy biomarkers for cutaneous melanoma. Front Oncol 6:236. https://doi.org/10.3389/fonc.2016.00236
doi: 10.3389/fonc.2016.00236
pubmed: 27882308
pmcid: 5101199
Kimura A, Ishida Y, Inagaki M, Nakamura Y, Sanke T, Mukaida N, Kondo T (2012) Interferon-γ is protective in cisplatin-induced renal injury by enhancing autophagic flux. Kidney Int 82:1093–1104
doi: 10.1038/ki.2012.240
Liu WJ, Ye L, Huang WF, Guo LJ, Xu ZG, Wu HL, Yang C, Liu HF (2016) p62 links the autophagy pathway and the ubiqutin-proteasome system upon ubiquitinated protein degradation. Cell Mol Biol Lett 21:29. https://doi.org/10.1186/s11658-016-0031-z
doi: 10.1186/s11658-016-0031-z
pubmed: 28536631
pmcid: 5415757
Caccamo A, Ferreira E, Branca C, Oddo S (2017) p62 improves AD-like pathology by increasing autophagy. Mol Psychiatry 22:865–873
doi: 10.1038/mp.2016.139
Salminen A, Kaarniranta K, Haapasalo A, Hiltunen M, Soininen H, Alafuzoff I (2012) Emerging role of p62/sequestosome-1 in the pathogenesis of Alzheimer’s disease. Prog Neurobiol 96:87–95
doi: 10.1016/j.pneurobio.2011.11.005
Odagiri S, Tanji K, Mori F, Kakita A, Takahashi H, Wakabayashi K (2012) Autophagic adapter protein NBR1 is localized in Lewy bodies and glial cytoplasmic inclusions and is involved in aggregate formation in α-synucleinopathy. Acta Neuropathol 124:173–186
doi: 10.1007/s00401-012-0975-7
Tanji K, Maruyama A, Odagiri S, Mori F, Itoh K, Kakita A, Takahashi H, Wakabayashi K (2013) Keap1 is localized in neuronal and glial cytoplasmic inclusions in various neurodegenerative diseases. J Neuropathol Exp Neurol 72:18–28
doi: 10.1097/NEN.0b013e31827b5713
Konstantinides SV (2014) 2014 ESC Guidelines on the diagnosis and management of acute pulmonary embolism. Eur Heart J 35:3145–3146. https://doi.org/10.1093/eurheartj/ehu393
doi: 10.1093/eurheartj/ehu393
pubmed: 25452462
Nosaka M, Ishida Y, Kimura A, Kuninaka Y, Inui M, Mukaida N, Kondo T (2011) Absence of IFN-γ accelerates thrombus resolution through enhanced MMP-9 and VEGF expression in mice. J Clin Invest 121:2911–2920. https://doi.org/10.1172/JCI40782
doi: 10.1172/JCI40782
pubmed: 21646723
pmcid: 3223815
Nosaka M, Ishida Y, Kimura A, Kondo T (2013) Immunohistochemical detection of intrathrombotic macrophage-derived cytokines and its application to thrombus age estimation in murine deep vein thrombosis model. Int J Legal Med 127:937–942. https://doi.org/10.1007/s00414-013-0873-0
doi: 10.1007/s00414-013-0873-0
Nosaka M, Ishida Y, Kimura A, Hama M, Kawaguchi T, Yamamoto H, Kuninaka Y, Shimada E, Kondo T (2015) Immunohistochemical detection of intrathrombotic IL-6 and its application to thrombus age estimation. Int J Legal Med 129:1021–1025. https://doi.org/10.1007/s00414-015-1147-9
doi: 10.1007/s00414-015-1147-9
Nosaka M, Ishida Y, Kimura A, Kondo T (2009) Time-dependent appearance of intrathrombus neutrophils and macrophages in a stasis-induced deep vein thrombosis model and its application to thrombus age determination. Int J Legal Med 123:235–240. https://doi.org/10.1007/s00414-009-0324-0
doi: 10.1007/s00414-009-0324-0
Nosaka M, Ishida Y, Kimura A, Kondo T (2010) Immunohistochemical detection of MMP-2 and MMP-9 in a stasis-induced deep vein thrombosis model and its application to thrombus age estimation. Int J Legal Med 124:439–444. https://doi.org/10.1007/s00414-010-0484-y
doi: 10.1007/s00414-010-0484-y
pubmed: 20623133
pmcid: 20623133
Nosaka M, Ishida Y, Kimura A, Kondo T (2010) Time-dependent organic changes of intravenous thrombi in stasis-induced deep vein thrombosis model and its application to thrombus age determination. Forensic Sci Int 195:143–147. https://doi.org/10.1016/j.forsciint.2009.12.008
doi: 10.1016/j.forsciint.2009.12.008
Nosaka M, Ishida Y, Kuninaka Y, Kimura A, Kondo T (2012) Immunohistochemical detection of uPA, tPA, and PAI-1 in a stasis-induced deep vein thrombosis model and its application to thrombus age estimation. Int J Legal Med 126:421–425. https://doi.org/10.1007/s00414-012-0680-z
doi: 10.1007/s00414-012-0680-z
Nosaka M, Ishida Y, Kimura A, Kawaguchi T, Yamamoto H, Kuninaka Y, Kondo T (2016) Immunohistochemical detection of intrathrombotic fibrocytes and its application to thrombus age estimation in murine deep vein thrombosis model. Int J Legal Med 131:179–183. https://doi.org/10.1007/s00414-016-1465-6
doi: 10.1007/s00414-016-1465-6
pubmed: 27757576
Cecchi R, Lazzaro A, Catanese M, Mandarelli G, Ferracuti S (2012) Fatal thromboembolism following physical restraint in a patient with schizophrenia. Int J Legal Med 126:477–482. https://doi.org/10.1007/s00414-012-0670-1
doi: 10.1007/s00414-012-0670-1
Brandimarti F, Alessandrini F, Pesaresi M, Catalani C, De Angelis L, Galeazzi R, Giovagnetti S, Gesuita R, Righi E, Giorgetti R, Tagliabracci A (2017) Investigation on genetic thrombophilic factors in FFPE autopsy tissue from subjects who died from pulmonary embolism. Int J Legal Med 131:447–458. https://doi.org/10.1007/s00414-016-1508-z
doi: 10.1007/s00414-016-1508-z
Foschi N, Ragonese M, Grassi VM, De Matteis V, De-Giorgio F (2017) The periprostatic venous plexus: an unusual source of fatal pulmonary embolism during corporoplasty. Int J Legal Med 131:713–717. https://doi.org/10.1007/s00414-016-1519-9
doi: 10.1007/s00414-016-1519-9
pubmed: 28004195
Goldhaber SZ, Bounameaux H (2012) Pulmonary embolism and deep vein thrombosis. Lancet 379:1835–1846. https://doi.org/10.1016/S0140-6736(11)61904-1
doi: 10.1016/S0140-6736(11)61904-1
pubmed: 22494827
Irninger W (1963) Histologische Altersbestimmung von Thrombosen und Embolien. Virchows Arch Pathol Anat 336:220–237
doi: 10.1007/BF00957911
Kondo T, Ishida Y (2010) Molecular pathology of wound healing. Forensic Sci Int 203:93–98. https://doi.org/10.1016/j.forsciint.2010.07.004
doi: 10.1016/j.forsciint.2010.07.004
pubmed: 20739128
pmcid: 20739128
Kondo T, Ohshima T (1996) The dynamics of inflammatory cytokines in the healing process of mouse skin wound: a preliminary study for possible wound age determination. Int J Legal Med 108:231–236
doi: 10.1007/BF01369816
Kondo T (2007) Timing of skin wounds. Leg Med (Tokyo) 9:109–114. https://doi.org/10.1016/j.legalmed.2006.11.009
doi: 10.1016/j.legalmed.2006.11.009
Ishida Y, Kimura A, Takayasu T, Eisenmenger W, Kondo T (2009) Detection of fibrocytes in human skin wounds and its application for wound age determination. Int J Legal Med 123:299–304. https://doi.org/10.1007/s00414-009-0320-4
doi: 10.1007/s00414-009-0320-4
Ishida Y, Kimura A, Nosaka M, Kuninaka Y, Shimada E, Yamamoto H, Nishiyama K, Inaka S, Takayasu T, Eisenmenger W, Kondo T (2015) Detection of endothelial progenitor cells in human skin wounds and its application for wound age determination. Int J Legal Med 129:1049–1054. https://doi.org/10.1007/s00414-015-1181-7
doi: 10.1007/s00414-015-1181-7
Cecchi R, Sestili C, Prosperini G, Cecchetto G, Vicini E, Viel G, Muciaccia B (2014) Markers of mechanical asphyxia: immunohistochemical study on autoptic lung tissues. Int J Legal Med 128:117–125. https://doi.org/10.1007/s00414-013-0876-x
doi: 10.1007/s00414-013-0876-x
Mizushima N, Komatsu M (2011) Autophagy: renovation of cells and tissues. Cell 147:728–741. https://doi.org/10.1016/j.cell.2011.10.026
doi: 10.1016/j.cell.2011.10.026
pubmed: 22078875
Komatsu M, Ichimura Y (2010) Physiological significance of selective degradation of p62 by autophagy. FEBS Lett 584:1374–1378. https://doi.org/10.1016/j.febslet.2010.02.017
doi: 10.1016/j.febslet.2010.02.017
pubmed: 20153326
Nosaka M, Ishida Y, Kimura A, Kuninaka Y, Taruya A, Furuta M, Mukaida N, Kondo T (2018) Contribution of the TNF-α (tumor necrosis factor-α)-TNF-Rp55 (tumor necrosis factor receptor p55) axis in the resolution of venous thrombus. Arterioscler Thromb Vasc Biol 38:2638–2650. https://doi.org/10.1161/ATVBAHA.118.311194
doi: 10.1161/ATVBAHA.118.311194
pubmed: 30354252
Zhang X, Jin JY, Wu J, Qin X, Streilein R, Hall RP, Zhang JY (2015) RNA-Seq and ChIP-Seq reveal SQSTM1/p62 as a key mediator of JunB suppression of NF-κB-dependent inflammation. J Investig Dermatol 135:1016–1024. https://doi.org/10.1038/jid.2014.519
doi: 10.1038/jid.2014.519
pubmed: 25501661
Joosten LA, Netea MG, Dinarello CA (2013) Interleukin-1β in innate inflammation, autophagy and immunity. Semin Immunol 25:416–424. https://doi.org/10.1016/j.smim.2013.10.018
doi: 10.1016/j.smim.2013.10.018
pubmed: 24275601
Chang CP, Su YC, Lee PH, Lei HY (2013) Targeting NFκB by autophagy to polarize hepatoma-associated macrophage differentiation. Autophagy 9:619–621. https://doi.org/10.4161/auto.23546
doi: 10.4161/auto.23546
pubmed: 23360732
pmcid: 3627680