MiR-21 Promotes the Invasion and Metastasis of Gastric Cancer Cells by Activating Epithelial-Mesenchymal Transition.
Epithelial-mesenchymal transition
Gastric cancer
Invasion
Metastasis
miRNA-21
Journal
European surgical research. Europaische chirurgische Forschung. Recherches chirurgicales europeennes
ISSN: 1421-9921
Titre abrégé: Eur Surg Res
Pays: Switzerland
ID NLM: 0174752
Informations de publication
Date de publication:
2019
2019
Historique:
received:
02
05
2019
accepted:
15
10
2019
pubmed:
14
11
2019
medline:
19
6
2020
entrez:
14
11
2019
Statut:
ppublish
Résumé
Gastric cancer (GC) is one of the most common malignant tumors. It is likely to occur in lymph nodes and is prone to distant metastasis in its early stages, which portends a poor prognosis. Previous studies have shown that miRNA-21 was abnormally highly expressed and associated with early metastasis in GC, but the mechanism by which it regulates the invasion and metastasis of GC has not been elucidated. Epithelial-mesenchymal transition (EMT) is an important pathologic basis of tumor invasion and metastasis, and in this study, the relationship between miRNA-21 and EMT in GC invasion and metastasis was investigated using RT-qPCR, Western blot, and wound scratch and transwell assays. We found that miRNA-21 expression in GC cell lines was higher than in a gastric mucosal epithelial cell line. After transfection with an miRNA-21 mimic, the upregulation of EMT was found to promote migration and invasion of MGC-803 cells. However, the downregulation of EMT was found to accompany the inhibition of invasion and migration of GC cells after downregulation of miRNA-21 expression due to the transfection of an miRNA-21 inhibitor. These findings suggest that miRNA-21 might promote the invasion and metastasis of GC by upregulating EMT.
Sections du résumé
BACKGROUND
BACKGROUND
Gastric cancer (GC) is one of the most common malignant tumors. It is likely to occur in lymph nodes and is prone to distant metastasis in its early stages, which portends a poor prognosis. Previous studies have shown that miRNA-21 was abnormally highly expressed and associated with early metastasis in GC, but the mechanism by which it regulates the invasion and metastasis of GC has not been elucidated.
METHODS
METHODS
Epithelial-mesenchymal transition (EMT) is an important pathologic basis of tumor invasion and metastasis, and in this study, the relationship between miRNA-21 and EMT in GC invasion and metastasis was investigated using RT-qPCR, Western blot, and wound scratch and transwell assays.
RESULTS
RESULTS
We found that miRNA-21 expression in GC cell lines was higher than in a gastric mucosal epithelial cell line. After transfection with an miRNA-21 mimic, the upregulation of EMT was found to promote migration and invasion of MGC-803 cells. However, the downregulation of EMT was found to accompany the inhibition of invasion and migration of GC cells after downregulation of miRNA-21 expression due to the transfection of an miRNA-21 inhibitor.
CONCLUSIONS
CONCLUSIONS
These findings suggest that miRNA-21 might promote the invasion and metastasis of GC by upregulating EMT.
Identifiants
pubmed: 31722341
pii: 000504133
doi: 10.1159/000504133
doi:
Substances chimiques
MIRN21 microRNA, human
0
MicroRNAs
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
208-218Informations de copyright
© 2019 S. Karger AG, Basel.