Extracellular microRNA 130b-3p inhibits eCIRP-induced inflammation.
eCIRP
inflammation
macrophages
miRNA 130b-3p
sepsis
Journal
EMBO reports
ISSN: 1469-3178
Titre abrégé: EMBO Rep
Pays: England
ID NLM: 100963049
Informations de publication
Date de publication:
07 01 2020
07 01 2020
Historique:
received:
12
03
2019
revised:
18
10
2019
accepted:
24
10
2019
pubmed:
15
11
2019
medline:
28
4
2021
entrez:
15
11
2019
Statut:
ppublish
Résumé
Although microRNAs regulate mRNA expression intracellularly, they are often released into the circulation in inflammatory diseases. During sepsis, secreted extracellular cold-inducible RNA-binding protein (eCIRP) acts as a damage-associated molecular pattern (DAMP), inducing tissue damage by elevating inflammatory cytokines and chemokines. Here, we report that the circulating microRNA 130b-3p inhibits eCIRP-mediated sterile and cecal ligation and puncture (CLP)-induced non-sterile inflammation. We find that levels of miR-130b-3p are increased in the serum of septic mice and patients and that it strongly interacts with recombinant murine (rm) CIRP in vitro and with eCIRP in the serum of septic mice in vivo. Combining a miR-130b-3p mimic with rmCIRP significantly decreases TNF-α release by macrophages compared to only rmCIRP-treated cells. This combined treatment also dose-dependently decreases the affinity of rmCIRP with its receptor TLR4/MD2. Finally, injection of a miR-130b-3p mimic significantly reduces rmCIRP- or CLP-induced systemic inflammation and acute lung injury in mice. These data show that extracellular miR-130b-3p functions as a novel endogenous inhibitor of eCIRP and point to an innovative therapeutic approach to treat inflammatory diseases.
Identifiants
pubmed: 31724825
doi: 10.15252/embr.201948075
pmc: PMC10563445
doi:
Substances chimiques
Cytokines
0
MIRN130 microRNA, human
0
MIRN130 microRNA, mouse
0
MicroRNAs
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
e48075Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM129633
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM118337
Pays : United States
Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2019 The Authors. Published under the terms of the CC BY 4.0 license.
Références
Gene. 1997 Dec 19;204(1-2):115-20
pubmed: 9434172
J Leukoc Biol. 2019 Jul;106(1):133-146
pubmed: 30645013
Drug Deliv. 2012 Nov;19(8):392-405
pubmed: 23173580
Int J Biochem Cell Biol. 2016 May;74:72-83
pubmed: 26923288
J Mol Biol. 2007 Sep 21;372(3):774-97
pubmed: 17681537
Diabetes Metab Res Rev. 2015 Oct;31(7):717-24
pubmed: 25952368
Nat Rev Mol Cell Biol. 2019 Jan;20(1):21-37
pubmed: 30108335
Front Immunol. 2015 Nov 20;6:588
pubmed: 26635802
Nat Rev Mol Cell Biol. 2014 Aug;15(8):509-24
pubmed: 25027649
Oncogene. 2012 Aug 16;31(33):3818-25
pubmed: 22120719
Adv Drug Deliv Rev. 2015 Jan;81:75-93
pubmed: 25220354
Cell. 2009 Feb 20;136(4):642-55
pubmed: 19239886
EMBO Mol Med. 2014 Jun 16;6(7):851-64
pubmed: 24935956
Cell Death Dis. 2017 Sep 7;8(9):e3045
pubmed: 28880270
Sci Rep. 2016 Feb 03;6:20475
pubmed: 26837280
Stroke. 2017 Jan;48(1):10-16
pubmed: 27899750
Nucleic Acids Res. 2008 Jul 1;36(Web Server issue):W265-9
pubmed: 18539611
Nucleic Acids Res. 2012 Aug;40(14):e112
pubmed: 22539264
Neoplasia. 2013 Sep;15(9):1086-99
pubmed: 24027433
Am J Respir Cell Mol Biol. 2011 May;44(5):725-38
pubmed: 21531958
Trends Biochem Sci. 2016 Oct;41(10):883-892
pubmed: 27597517
Nucleic Acids Res. 2014 Jan;42(Database issue):D68-73
pubmed: 24275495
PLoS One. 2008 Sep 05;3(9):e3148
pubmed: 18773077
Int J Mol Sci. 2018 Jun 13;19(6):
pubmed: 29899293
J Comput Chem. 2004 Oct;25(13):1605-12
pubmed: 15264254
Ann N Y Acad Sci. 2018 Jan;1412(1):33-40
pubmed: 29125182
Pediatr Blood Cancer. 2015 Aug;62(8):1360-7
pubmed: 25787821
Pathol Res Pract. 2018 Oct;214(10):1655-1660
pubmed: 30153957
J Immunol. 2017 Sep 15;199(6):2106-2117
pubmed: 28768728
PLoS One. 2013;8(2):e57426
pubmed: 23437386
Front Immunol. 2014 Sep 25;5:461
pubmed: 25309543
PLoS One. 2016 Mar 08;11(3):e0150418
pubmed: 26953888
Sci Rep. 2017 Feb 06;7:41942
pubmed: 28165066
Nat Methods. 2015 Jan;12(1):7-8
pubmed: 25549265
Sci Rep. 2016 Aug 18;6:32153
pubmed: 27535582
Trends Cell Biol. 2012 Mar;22(3):125-32
pubmed: 22260888
Front Immunol. 2017 Feb 16;8:118
pubmed: 28261201
PLoS One. 2013 Oct 30;8(10):e77936
pubmed: 24205035
Mol Ther Nucleic Acids. 2016 Oct 11;5(10):e373
pubmed: 27727249
Oncotarget. 2016 Jul 26;7(30):48547-48561
pubmed: 27391066
Nucleic Acids Res. 2015 Jul 1;43(W1):W425-30
pubmed: 25977296
Sci Rep. 2015 Aug 28;5:12644
pubmed: 26316103
Nat Med. 2013 Nov;19(11):1489-1495
pubmed: 24097189
Diagn Pathol. 2014 Aug 15;9:160
pubmed: 25123453
Proc Natl Acad Sci U S A. 2008 Jul 29;105(30):10513-8
pubmed: 18663219
Mol Med. 2010 Jan-Feb;16(1-2):69-82
pubmed: 19949486
J Leukoc Biol. 2013 Mar;93(3):329-42
pubmed: 23136259
EMBO Rep. 2020 Jan 7;21(1):e48075
pubmed: 31724825
J Leukoc Biol. 2007 Jan;81(1):1-5
pubmed: 17032697
J Clin Invest. 2001 Jan;107(1):7-11
pubmed: 11134171
Nucleic Acids Res. 2011 Sep 1;39(16):7223-33
pubmed: 21609964
J Cell Biol. 1997 May 19;137(4):899-908
pubmed: 9151692
Proc Natl Acad Sci U S A. 2011 Jul 12;108(28):11542-7
pubmed: 21709231