A novel therapeutic approach using peripheral blood mononuclear cells preconditioned by oxygen-glucose deprivation.
Animals
Brain
/ blood supply
Brain Ischemia
/ metabolism
Cells, Cultured
Disease Models, Animal
Glucose
/ pharmacology
Humans
Leukocytes, Mononuclear
/ cytology
Mice
Oxygen
/ pharmacology
Primary Cell Culture
Rats
Transforming Growth Factor beta
/ metabolism
Treatment Outcome
Vascular Endothelial Growth Factor A
/ metabolism
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
14 11 2019
14 11 2019
Historique:
received:
28
03
2019
accepted:
31
10
2019
entrez:
16
11
2019
pubmed:
16
11
2019
medline:
4
11
2020
Statut:
epublish
Résumé
Cell therapies that invoke pleiotropic mechanisms may facilitate functional recovery in patients with stroke. Based on previous experiments using microglia preconditioned by oxygen-glucose deprivation, we hypothesized that the administration of peripheral blood mononuclear cells (PBMCs) preconditioned by oxygen-glucose deprivation (OGD-PBMCs) to be a therapeutic strategy for ischemic stroke. Here, OGD-PBMCs were identified to secrete remodelling factors, including the vascular endothelial growth factor and transforming growth factor-β in vitro, while intra-arterial administration of OGD-PBMCs at 7 days after focal cerebral ischemia prompted expression of such factors in the brain parenchyma at 28 days following focal cerebral ischemia in vivo. Furthermore, administration of OGD-PBMCs induced an increasing number of stage-specific embryonic antigen-3-positive cells both in vitro and in vivo. Finally, it was found to prompt angiogenesis and axonal outgrowth, and functional recovery after cerebral ischemia. In conclusion, the administration of OGD-PBMCs might be a novel therapeutic strategy against ischemic stroke.
Identifiants
pubmed: 31728010
doi: 10.1038/s41598-019-53418-5
pii: 10.1038/s41598-019-53418-5
pmc: PMC6856386
doi:
Substances chimiques
Transforming Growth Factor beta
0
Vascular Endothelial Growth Factor A
0
Glucose
IY9XDZ35W2
Oxygen
S88TT14065
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
16819Commentaires et corrections
Type : ErratumIn
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