Derivation of self-renewing lung alveolar epithelial type II cells from human pluripotent stem cells.
Journal
Nature protocols
ISSN: 1750-2799
Titre abrégé: Nat Protoc
Pays: England
ID NLM: 101284307
Informations de publication
Date de publication:
12 2019
12 2019
Historique:
received:
12
01
2019
accepted:
27
06
2019
pubmed:
17
11
2019
medline:
8
2
2020
entrez:
17
11
2019
Statut:
ppublish
Résumé
Alveolar epithelial type II cells (AEC2s) are the facultative progenitors of lung alveoli and serve as the surfactant-producing cells of air-breathing organisms. Although primary human AEC2s are difficult to maintain stably in cell cultures, recent advances have facilitated the derivation of AEC2-like cells from human pluripotent stem cells (hPSCs) in vitro. Here, we provide a detailed protocol for the directed differentiation of hPSCs into self-renewing AEC2-like cells that can be maintained for up to 1 year in culture as epithelial-only spheres without the need for supporting mesenchymal feeder cells. The month-long protocol requires recapitulation of the sequence of milestones associated with in vivo development of the distal lung, beginning with differentiation of cells into anterior foregut endoderm, which is followed by their lineage specification into NKX2-1
Identifiants
pubmed: 31732721
doi: 10.1038/s41596-019-0220-0
pii: 10.1038/s41596-019-0220-0
pmc: PMC7275645
mid: NIHMS1584457
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3303-3332Subventions
Organisme : NCATS NIH HHS
ID : U01 TR001810
Pays : United States
Organisme : NHLBI NIH HHS
ID : R24 HL123828
Pays : United States
Organisme : NHLBI NIH HHS
ID : U01 HL134745
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL095993
Pays : United States
Organisme : NHLBI NIH HHS
ID : U01 HL134766
Pays : United States
Organisme : NHLBI NIH HHS
ID : U01 HL148692
Pays : United States
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