Molecular cloning and functional characterization of duck Janus kinase 1.

Animals Avian Proteins / genetics Ducks / genetics Immunity, Innate / genetics Janus Kinase 1 / genetics
Duck Innate immunity JAK1

Journal

Molecular immunology
ISSN: 1872-9142
Titre abrégé: Mol Immunol
Pays: England
ID NLM: 7905289

Informations de publication

Date de publication:
01 2020
Historique:
received: 25 07 2019
revised: 06 09 2019
accepted: 29 10 2019
pubmed: 17 11 2019
medline: 10 4 2020
entrez: 17 11 2019
Statut: ppublish

Résumé

Janus kinase 1 (JAK1) is a member of JAK family of non-receptor protein tyrosine kinases that plays critical roles in transducing cytokine signals via JAK-signal transducer and activator of transcription (STAT) signaling pathway. The importance of JAK1 in innate immunity has been well-studied in mammals and fish, yet in avian remains largely unknown. Here, we cloned the full-length of the duck JAK1 (duJAK1) gene for the first time. DuJAK1 encoded a protein of 1152 amino acids and possessed high amino acid identity with goose and budgerigar JAK1s. The duJAK1 was expressed in all detected tissues, especially high in the thymus and bursa of Fabricius. Overexpression of duJAK1 significantly activated ISRE promoter activity and induced duck viperin, 2', 5'-OAS, MX, PKR and ZAP expression. Knockdown of duJAK1 by small interfering RNA significantly inhibited duck Tembusu virus (DTMUV)-, duck Enteritis virus (DEV)-, poly (I:C)-, poly (dA:dT)- or Sendai virus (SeV)-induced ISRE promoter activation. Furthermore, duJAK1 exhibited antiviral activity against DTMUV infection. These results will help us understand the function of JAK family proteins in duck antiviral immunity.

Identifiants

DOI: 10.1016/j.molimm.2019.10.022 PMID: 31733446
pubmed: 31733446
pii: S0161-5890(19)30540-1
doi: 10.1016/j.molimm.2019.10.022
pii:
doi:

Substances chimiques

Avian Proteins 0
Janus Kinase 1 EC 2.7.10.2

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

29-36

Informations de copyright

Copyright © 2019 Elsevier Ltd. All rights reserved.

Auteurs

Dejian Liu (D)

State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, the Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei 430070, China.

Huijun Zheng (H)

State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, the Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei 430070, China.

Yaqian Li (Y)

State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, the Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei 430070, China.

Peng Zhou (P)

State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, the Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei 430070, China.

Hui Jin (H)

State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, the Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei 430070, China.

Rui Luo (R)

State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, the Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei 430070, China. Electronic address: luorui@mail.hzau.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Molecular cloning and functional...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines aviaires › Molecular cloning and functional...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Oiseaux › Canards › Molecular cloning and functional...
questionsmedicales.fr › Phénomènes du système immunitaire › Immunité › Immunité innée › Molecular cloning and functional...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Protein-tyrosine kinases › Janus kinases › Janus kinase 1 › Molecular cloning and functional...