Adult Drosophila Lack Hematopoiesis but Rely on a Blood Cell Reservoir at the Respiratory Epithelia to Relay Infection Signals to Surrounding Tissues.

Animals Blood Cells / metabolism Drosophila / metabolism Hematopoiesis / physiology Hemocytes / metabolism Immunity, Cellular / immunology Immunity, Innate / immunology Janus Kinases / metabolism Macrophages / metabolism Respiratory Mucosa / metabolism Transcription Factors / metabolism
Drosocin Drosophila melanogaster Jak/Stat NFκB Stat92E antimicrobial peptide blood cell reservoir fat body hematopoiesis hemocyte hop imd innate immunity macrophage respiratory epithelia tracheal air sacs upd3

Journal

Developmental cell
ISSN: 1878-1551
Titre abrégé: Dev Cell
Pays: United States
ID NLM: 101120028

Informations de publication

Date de publication:
16 12 2019
Historique:
received: 16 03 2019
revised: 08 08 2019
accepted: 17 10 2019
pubmed: 19 11 2019
medline: 11 6 2020
entrez: 19 11 2019
Statut: ppublish

Résumé

The use of adult Drosophila melanogaster as a model for hematopoiesis or organismal immunity has been debated. Addressing this question, we identify an extensive reservoir of blood cells (hemocytes) at the respiratory epithelia (tracheal air sacs) of the thorax and head. Lineage tracing and functional analyses demonstrate that the majority of adult hemocytes are phagocytic macrophages (plasmatocytes) from the embryonic lineage that parallels vertebrate tissue macrophages. Surprisingly, we find no sign of adult hemocyte expansion. Instead, hemocytes play a role in relaying an innate immune response to the blood cell reservoir: through Imd signaling and the Jak/Stat pathway ligand Upd3, hemocytes act as sentinels of bacterial infection, inducing expression of the antimicrobial peptide Drosocin in respiratory epithelia and colocalizing fat body domains. Drosocin expression in turn promotes animal survival after infection. Our work identifies a multi-signal relay of organismal humoral immunity, establishing adult Drosophila as model for inter-organ immunity.

Identifiants

DOI: 10.1016/j.devcel.2019.10.017 PMID: 31735669 PMC: PMC7263735
pubmed: 31735669
pii: S1534-5807(19)30864-0
doi: 10.1016/j.devcel.2019.10.017
pmc: PMC7263735
mid: NIHMS1543869
pii:
doi:

Substances chimiques

Transcription Factors 0
Janus Kinases EC 2.7.10.2

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

787-803.e5

Subventions

Organisme : NIGMS NIH HHS
ID : R01 GM112083
Pays : United States
Organisme : NHLBI NIH HHS
ID : R56 HL118726
Pays : United States
Organisme : NCRR NIH HHS
ID : C06 RR016490
Pays : United States
Organisme : Wellcome Trust
ID : WT101853/C/13/Z
Pays : United Kingdom
Organisme : NIAID NIH HHS
ID : R01 AI130345
Pays : United States
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : NCI NIH HHS
ID : P30 CA008748
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM131094
Pays : United States

Informations de copyright

Copyright © 2019 Elsevier Inc. All rights reserved.

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Auteurs

Pablo Sanchez Bosch (P)

Department of Cell and Tissue Biology, University of California, San Francisco, San Francisco, CA, USA.

Kalpana Makhijani (K)

Department of Cell and Tissue Biology, University of California, San Francisco, San Francisco, CA, USA.

Leire Herboso (L)

Department of Cell and Tissue Biology, University of California, San Francisco, San Francisco, CA, USA.

Katrina S Gold (KS)

Department of Cell and Tissue Biology, University of California, San Francisco, San Francisco, CA, USA.

Rowan Baginsky (R)

Department of Cell and Tissue Biology, University of California, San Francisco, San Francisco, CA, USA.

Katie J Woodcock (KJ)

King's College London, London, UK.

Brandy Alexander (B)

Department of Cell and Tissue Biology, University of California, San Francisco, San Francisco, CA, USA.

Katelyn Kukar (K)

Department of Cell and Tissue Biology, University of California, San Francisco, San Francisco, CA, USA.

Sean Corcoran (S)

Department of Cell and Tissue Biology, University of California, San Francisco, San Francisco, CA, USA.

Thea Jacobs (T)

Department of Cell and Tissue Biology, University of California, San Francisco, San Francisco, CA, USA.

Debra Ouyang (D)

Department of Cell and Tissue Biology, University of California, San Francisco, San Francisco, CA, USA.

Corinna Wong (C)

Department of Cell and Tissue Biology, University of California, San Francisco, San Francisco, CA, USA.

Elodie J V Ramond (EJV)

EPFL, Lausanne, Switzerland.

Christa Rhiner (C)

Champalimaud Center for the Unknown, Lisbon, Portugal.

Eduardo Moreno (E)

Champalimaud Center for the Unknown, Lisbon, Portugal.

Bruno Lemaitre (B)

EPFL, Lausanne, Switzerland.

Frederic Geissmann (F)

King's College London, London, UK; Memorial Sloan Kettering Cancer Center, New York, NY, USA.

Katja Brückner (K)

Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, San Francisco, CA, USA; Department of Cell and Tissue Biology, University of California, San Francisco, San Francisco, CA, USA; Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA, USA. Electronic address: katja.brueckner@ucsf.edu.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Adult Drosophila Lack Hematopoiesis but Rely...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Sang › Cellules sanguines › Adult Drosophila Lack Hematopoiesis but Rely...
questionsmedicales.fr › Eucaryotes › Animaux › Invertébrés › Arthropodes › Insectes › Pterygota › Neoptera › Holometabola › Diptera › Drosophilidae › Drosophila › Adult Drosophila Lack Hematopoiesis but Rely...
questionsmedicales.fr › Phénomènes physiologiques respiratoires et circulatoires › Phénomènes physiogiques du sang › Hématopoïèse › Adult Drosophila Lack Hematopoiesis but Rely...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Sang › Cellules sanguines › Hémocytes › Adult Drosophila Lack Hematopoiesis but Rely...
questionsmedicales.fr › Phénomènes du système immunitaire › Immunité › Immunité acquise › Immunité cellulaire › Adult Drosophila Lack Hematopoiesis but Rely...
questionsmedicales.fr › Phénomènes du système immunitaire › Immunité › Immunité innée › Adult Drosophila Lack Hematopoiesis but Rely...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Protein-tyrosine kinases › Janus kinases › Adult Drosophila Lack Hematopoiesis but Rely...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Phagocytes › Macrophages › Adult Drosophila Lack Hematopoiesis but Rely...
questionsmedicales.fr › Tissus › Membranes › Muqueuse › Muqueuse respiratoire › Adult Drosophila Lack Hematopoiesis but Rely...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › Adult Drosophila Lack Hematopoiesis but Rely...