Vaccine-Induced Skewing of T Cell Responses Protects Against Chikungunya Virus Disease.
Chikungunya virus (CHIKV)
T cell
cytokine
pathogenesis
vaccine
Journal
Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960
Informations de publication
Date de publication:
2019
2019
Historique:
received:
07
08
2019
accepted:
16
10
2019
entrez:
19
11
2019
pubmed:
19
11
2019
medline:
21
10
2020
Statut:
epublish
Résumé
Chikungunya virus (CHIKV) infections can cause severe and debilitating joint and muscular pain that can be long lasting. Current CHIKV vaccines under development rely on the generation of neutralizing antibodies for protection; however, the role of T cells in controlling CHIKV infection and disease is still unclear. Using an overlapping peptide library, we identified the CHIKV-specific T cell receptor epitopes recognized in C57BL/6 infected mice at 7 and 14 days post-infection. A fusion protein containing peptides 451, 416, a small region of nsP4, peptide 47, and an HA tag (CHKVf5) was expressed using adenovirus and cytomegalovirus-vectored vaccines. Mice vaccinated with CHKVf5 elicited robust T cell responses to higher levels than normally observed following CHIKV infection, but the vaccine vectors did not elicit neutralizing antibodies. CHKVf5-vaccinated mice had significantly reduced infectious viral load when challenged by intramuscular CHIKV injection. Depletion of both CD4
Identifiants
pubmed: 31736977
doi: 10.3389/fimmu.2019.02563
pmc: PMC6834551
doi:
Substances chimiques
Viral Vaccines
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
2563Subventions
Organisme : NIAID NIH HHS
ID : R41 AI138964
Pays : United States
Organisme : NIAID NIH HHS
ID : R41AI138964-01
Pays : United States
Organisme : NIAID NIH HHS
ID : T32 AI007472
Pays : United States
Organisme : NIAID NIH HHS
ID : U19 AI142759
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI108725
Pays : United States
Informations de copyright
Copyright © 2019 Broeckel, Haese, Ando, Dmitriev, Kreklywich, Powers, Denton, Smith, Morrison, Heise, DeFilippis, Messaoudi, Curiel and Streblow.
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