Expanding the CRISPR Toolbox with ErCas12a in Zebrafish and Human Cells.
Animals
Base Sequence
CRISPR-Associated Proteins
/ genetics
CRISPR-Cas Systems
/ genetics
Clustered Regularly Interspaced Short Palindromic Repeats
/ genetics
DNA
/ chemistry
Gene Editing
/ methods
Gene Targeting
/ methods
Genetic Engineering
/ methods
Genome
/ genetics
Humans
RNA
/ chemistry
RNA, Guide, Kinetoplastida
/ chemistry
Zebrafish
/ genetics
Journal
The CRISPR journal
ISSN: 2573-1602
Titre abrégé: CRISPR J
Pays: United States
ID NLM: 101738191
Informations de publication
Date de publication:
12 2019
12 2019
Historique:
pubmed:
20
11
2019
medline:
21
7
2020
entrez:
20
11
2019
Statut:
ppublish
Résumé
CRISPR and CRISPR-Cas effector proteins enable the targeting of DNA double-strand breaks to defined loci based on a variable length RNA guide specific to each effector. The guide RNAs are generally similar in size and form, consisting of a ∼20 nucleotide sequence complementary to the DNA target and an RNA secondary structure recognized by the effector. However, the effector proteins vary in protospacer adjacent motif requirements, nuclease activities, and DNA binding kinetics. Recently, ErCas12a, a new member of the Cas12a family, was identified in
Identifiants
pubmed: 31742435
doi: 10.1089/crispr.2019.0026
pmc: PMC6919245
doi:
Substances chimiques
CRISPR-Associated Proteins
0
RNA, Guide
0
RNA
63231-63-0
DNA
9007-49-2
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
417-433Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM063904
Pays : United States
Organisme : NIH HHS
ID : R24 OD020166
Pays : United States
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