Irisin increases the expression of anorexigenic and neurotrophic genes in mouse brain.
Agouti-Related Protein
/ genetics
Animals
Brain
/ drug effects
Brain-Derived Neurotrophic Factor
/ genetics
Fibronectins
/ pharmacology
Ghrelin
/ blood
Leptin
/ blood
Male
Mice
Nerve Tissue Proteins
/ genetics
Neuropeptide Y
/ genetics
Orexins
/ genetics
Pro-Opiomelanocortin
/ genetics
Uncoupling Protein 2
/ genetics
appetite regulators
food intake
ghrelin
irisin
leptin
Journal
Diabetes/metabolism research and reviews
ISSN: 1520-7560
Titre abrégé: Diabetes Metab Res Rev
Pays: England
ID NLM: 100883450
Informations de publication
Date de publication:
03 2020
03 2020
Historique:
received:
16
06
2019
revised:
13
10
2019
accepted:
11
11
2019
pubmed:
20
11
2019
medline:
21
1
2021
entrez:
20
11
2019
Statut:
ppublish
Résumé
Irisin, a newly discovered muscle-derived hormone, acts in different organs and tissues, improving energy homeostasis. In this study, we assessed, for the first time, the effects of intraperitoneal irisin injections on circulating levels of leptin and ghrelin, mRNA expression of the major hypothalamic appetite regulators and brain neurotrophic factors, as well as feeding behaviour in healthy mice. Twelve male 6-week-old C57BL/6 mice were randomized into two groups and intraperitoneally injected daily with irisin (0.5 μg/g body weight) or vehicle (phosphate-buffered saline [PBS]) for 14 days. On the last day of observation, leptin and ghrelin levels were measured with an enzyme-linked immunosorbent assay (ELISA). mRNA levels of genes of interest were analysed by quantitative reverse transcription polymerase chain reaction (qRT-PCR) in brain extracts. Irisin administration did not change leptin or ghrelin serum concentrations. However, irisin injection increased CART, POMC, NPY, and BDNF mRNA levels, without affecting the mRNA expression of AgRP, orexin, PMCH, and UCP2. Finally, over the time frame of irisin treatment, body weight and feeding behaviour were unaltered. These results suggest that intraperitoneal injection of irisin, although without effects on feeding behaviour and body weight, can increase the expression of anorexigenic and neurotrophic genes in mouse brain.
Sections du résumé
BACKGROUND
Irisin, a newly discovered muscle-derived hormone, acts in different organs and tissues, improving energy homeostasis. In this study, we assessed, for the first time, the effects of intraperitoneal irisin injections on circulating levels of leptin and ghrelin, mRNA expression of the major hypothalamic appetite regulators and brain neurotrophic factors, as well as feeding behaviour in healthy mice.
METHODS
Twelve male 6-week-old C57BL/6 mice were randomized into two groups and intraperitoneally injected daily with irisin (0.5 μg/g body weight) or vehicle (phosphate-buffered saline [PBS]) for 14 days. On the last day of observation, leptin and ghrelin levels were measured with an enzyme-linked immunosorbent assay (ELISA). mRNA levels of genes of interest were analysed by quantitative reverse transcription polymerase chain reaction (qRT-PCR) in brain extracts.
RESULTS
Irisin administration did not change leptin or ghrelin serum concentrations. However, irisin injection increased CART, POMC, NPY, and BDNF mRNA levels, without affecting the mRNA expression of AgRP, orexin, PMCH, and UCP2. Finally, over the time frame of irisin treatment, body weight and feeding behaviour were unaltered.
CONCLUSIONS
These results suggest that intraperitoneal injection of irisin, although without effects on feeding behaviour and body weight, can increase the expression of anorexigenic and neurotrophic genes in mouse brain.
Substances chimiques
Agouti-Related Protein
0
Agrp protein, mouse
0
Brain-Derived Neurotrophic Factor
0
FNDC5 protein, rat
0
Fibronectins
0
Ghrelin
0
Leptin
0
Nerve Tissue Proteins
0
Neuropeptide Y
0
Orexins
0
Uncoupling Protein 2
0
cocaine- and amphetamine-regulated transcript protein
0
Pro-Opiomelanocortin
66796-54-1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e3238Informations de copyright
© 2019 John Wiley & Sons, Ltd.
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