Designing a QTL Mapping Study for Implementation in the Realized Collaborative Cross Genetic Reference Population.
Collaborative Cross mouse model
complex traits
experimental design for QTL mapping
genetic reference population
quantitative trait loci
statistical power
Journal
Current protocols in mouse biology
ISSN: 2161-2617
Titre abrégé: Curr Protoc Mouse Biol
Pays: United States
ID NLM: 101560384
Informations de publication
Date de publication:
Dec 2019
Dec 2019
Historique:
entrez:
23
11
2019
pubmed:
23
11
2019
medline:
7
3
2020
Statut:
ppublish
Résumé
The Collaborative Cross (CC) mouse resource is a next-generation mouse genetic reference population (GRP) designed for high-resolution mapping of quantitative trait loci (QTL) of large effect affecting complex traits during health and disease. The CC resource consists of a set of 72 recombinant inbred lines (RILs) generated by reciprocal crossing of five classical and three wild-derived mouse founder strains. Complex traits are controlled by variations within multiple genes and environmental factors, and their mutual interactions. These traits are observed at multiple levels of the animals' systems, including metabolism, body weight, immune profile, and susceptibility or resistance to the development and progress of infectious or chronic diseases. Herein, we present general guidelines for design of QTL mapping experiments using the CC resource-along with full step-by-step protocols and methods that were implemented in our lab for the phenotypic and genotypic characterization of the different CC lines-for mapping the genes underlying host response to infectious and chronic diseases. © 2019 by John Wiley & Sons, Inc. Basic Protocol 1: CC lines for whole body mass index (BMI) Basic Protocol 2: A detailed assessment of the power to detect effect sizes based on the number of lines used, and the number of replicates per line Basic Protocol 3: Obtaining power for QTL with given target effect by interpolating in Table 1 of Keele et al. (2019).
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e66Informations de copyright
© 2019 John Wiley & Sons, Inc.
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