Dynamics of tumor-specific cfDNA in response to therapy in multiple myeloma patients.
cell-free DNA
liquid biopsy
multiple myeloma
qPCR
Journal
European journal of haematology
ISSN: 1600-0609
Titre abrégé: Eur J Haematol
Pays: England
ID NLM: 8703985
Informations de publication
Date de publication:
Mar 2020
Mar 2020
Historique:
received:
13
07
2019
revised:
12
11
2019
accepted:
13
11
2019
pubmed:
26
11
2019
medline:
8
10
2020
entrez:
26
11
2019
Statut:
ppublish
Résumé
Progress in multiple myeloma treatment allows patients to achieve deeper responses, for which the assessment of minimal residual disease (MRD) is critical. Typically, bone marrow samples are used for this purpose; however, this approach is site-limited. Liquid biopsy represents a minimally invasive and more comprehensive technique that is not site-limited, but equally challenging. While majority of current data comes from short-term studies, we present a long-term study on blood-based MRD monitoring using tumor-specific cell-free DNA detection by ASO-qPCR. One hundred and twelve patients were enrolled into the study, but long-term sampling and analysis were feasible only in 45 patients. We found a significant correlation of quantity of tumor-specific cell-free DNA levels with clinically meaningful events [induction therapy (P = .004); ASCT (P = .012)]. Moreover, length of cfDNA fragments is associated with better treatment response of patients. These results support the concept of tumor-specific cell-free DNA as a prognostic marker.
Identifiants
pubmed: 31763708
doi: 10.1111/ejh.13358
pmc: PMC7065130
doi:
Substances chimiques
Biomarkers, Tumor
0
Circulating Tumor DNA
0
Immunoglobulin Heavy Chains
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
190-197Subventions
Organisme : Ministerstvo Zdravotnictví Ceské Republiky
ID : AZV 17-29343A
Informations de copyright
© 2019 The Authors. European Journal of Haematology published by John Wiley & Sons Ltd.
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