Rough Brucella neotomae provides protection against Brucella suis challenge in mice.
Brucella neotomae
Immunity
Lipopolysaccharide
Live-attenuated vaccine
Rough phenotype
wboA glycosyltransferase
Journal
Veterinary microbiology
ISSN: 1873-2542
Titre abrégé: Vet Microbiol
Pays: Netherlands
ID NLM: 7705469
Informations de publication
Date de publication:
Dec 2019
Dec 2019
Historique:
received:
21
05
2019
revised:
26
07
2019
accepted:
03
10
2019
entrez:
27
11
2019
pubmed:
27
11
2019
medline:
12
2
2020
Statut:
ppublish
Résumé
Brucellosis is one of the most common zoonotic diseases worldwide. Almost 500,000 new human cases occur each year; yet there is no vaccine for human use. Moreover, there is no universal Brucella vaccine that would provide protection against all pathogenic species of Brucella. We generated a rough, live-attenuated B. neotomae strain by deleting the wboA gene encoding a glycosyltransferase. This strain lacks the O-side chain in its lipopolysaccharide (LPS) and thus the vaccinated animals can be differentiated serologically from the field-infected animals. We tested the efficacy of rough B. neotomae strain to stimulate dendritic cells compared to the smooth wild type strain. Based on TNF-α production, our data suggests that a significantly higher stimulation was obtained when dendritic cells were stimulated with the rough vaccine strain compared to the smooth wild type B. neotomae. Furthermore, the rough mutant was cleared from mice within 6 weeks even at a dose as high as 2 x 10
Identifiants
pubmed: 31767087
pii: S0378-1135(19)30605-4
doi: 10.1016/j.vetmic.2019.108447
pii:
doi:
Substances chimiques
Antibodies, Bacterial
0
Bacterial Proteins
0
Brucella Vaccine
0
Vaccines, Attenuated
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
108447Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.