Comparison of protein expression profile of limb regeneration between neotenic and metamorphic axolotl.
Axolotl
Gene expression profile
Limb regeneration
Metamorphosis
Proteomics
Journal
Biochemical and biophysical research communications
ISSN: 1090-2104
Titre abrégé: Biochem Biophys Res Commun
Pays: United States
ID NLM: 0372516
Informations de publication
Date de publication:
05 02 2020
05 02 2020
Historique:
received:
07
11
2019
accepted:
18
11
2019
pubmed:
27
11
2019
medline:
13
8
2020
entrez:
27
11
2019
Statut:
ppublish
Résumé
The axolotl (Ambystoma mexicanum) salamander, a urodele amphibian, has an exceptional regenerative capacity to fully restore an amputated limb throughout the life-long lasting neoteny. By contrast, when axolotls are experimentally induced to metamorphosis, attenuation of the limb's regenerative competence is noticeable. Here, we sought to discern the proteomic profiles of the early stages of blastema formation of neotenic and metamorphic axolotls after limb amputation by employing LC-MS/MS technology. We quantified a total of 714 proteins and qRT-PCR for selected genes was performed to validate the proteomics results and provide evidence for the putative link between immune system activity and regenerative potential. This study provides new insights for examination of common and distinct molecular mechanisms in regeneration-permissive neotenic and regeneration-deficient metamorphic stages at the proteome level.
Identifiants
pubmed: 31767146
pii: S0006-291X(19)32246-6
doi: 10.1016/j.bbrc.2019.11.118
pii:
doi:
Substances chimiques
Proteome
0
Types de publication
Comparative Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
428-434Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.