Purification and Reconstitution of the S. cerevisiae TRAMP and Ski Complexes for Biochemical and Structural Studies.
Air2
Exosome
Mtr4
Ski complex
Ski2
Ski3
Ski8
TRAMP
Trf4
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2020
2020
Historique:
entrez:
27
11
2019
pubmed:
27
11
2019
medline:
15
12
2020
Statut:
ppublish
Résumé
The RNA exosome is a macromolecular machine that degrades a large variety of RNAs from their 3'-end. It comprises the major 3'-to-5' exonuclease in the cell, completely degrades erroneous and overly abundant RNAs, and is also involved in the precise processing of RNAs. To degrade transcripts both specifically and efficiently the exosome functions together with compartment-specific cofactors. In the yeast S. cerevisiae, the exosome associates with the Ski complex in the cytoplasm and with Mtr4 alone or with Mtr4 as part of the TRAMP complex in the nucleus. Here we describe how to produce, purify, and assemble the Ski and TRAMP complexes from S. cerevisiae.
Identifiants
pubmed: 31768992
doi: 10.1007/978-1-4939-9822-7_24
doi:
Substances chimiques
RNA, Fungal
0
Saccharomyces cerevisiae Proteins
0
RNA
63231-63-0
Serine Endopeptidases
EC 3.4.21.-
tunicate retinoic acid-inducible modular protease
EC 3.4.21.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
491-513Commentaires et corrections
Type : ErratumIn
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