An isotope dilution LC-MS/MS based candidate reference method for the quantification of cyclosporine A, tacrolimus, sirolimus and everolimus in human whole blood.
Carbon Isotopes
/ chemistry
Chromatography, Liquid
/ methods
Cyclosporine
/ blood
Data Accuracy
Diagnostic Tests, Routine
/ methods
Drug Monitoring
/ methods
Everolimus
/ blood
Humans
Immunosuppressive Agents
/ blood
Indicator Dilution Techniques
Reference Standards
Sensitivity and Specificity
Sirolimus
/ blood
Solid Phase Extraction
/ methods
Tacrolimus
/ blood
Tandem Mass Spectrometry
/ methods
Immunosuppressive drugs
LC-MS/MS
Quantitative NMR
Reference measurement procedure
Whole blood
Journal
Clinical biochemistry
ISSN: 1873-2933
Titre abrégé: Clin Biochem
Pays: United States
ID NLM: 0133660
Informations de publication
Date de publication:
Aug 2020
Aug 2020
Historique:
received:
27
09
2019
revised:
07
11
2019
accepted:
08
11
2019
pubmed:
27
11
2019
medline:
20
1
2021
entrez:
27
11
2019
Statut:
ppublish
Résumé
An isotope dilution LC-MS/MS based candidate reference measurement procedure for the quantification of cyclosporine A, tacrolimus, sirolimus and everolimus in human whole blood is presented to be used for evaluation and standardization of routine assays applied for therapeutic drug monitoring. The assay allows baseline separation of the four immunosuppressive drugs within a total runtime of 9 minutes using a C4 reversed phase column. Sample preparation is based on protein precipitation with zinc sulphate followed by purification with solid phase extraction. Reference materials used in this reference measurement procedure were characterized by qNMR and an absolute content of analytes calculated to guarantee traceability to SI units. As internal standards the corresponding deuterated and
Identifiants
pubmed: 31770523
pii: S0009-9120(19)31063-X
doi: 10.1016/j.clinbiochem.2019.11.006
pii:
doi:
Substances chimiques
Carbon Isotopes
0
Immunosuppressive Agents
0
Cyclosporine
83HN0GTJ6D
Everolimus
9HW64Q8G6G
Sirolimus
W36ZG6FT64
Tacrolimus
WM0HAQ4WNM
Types de publication
Comparative Study
Journal Article
Validation Study
Langues
eng
Sous-ensembles de citation
IM
Pagination
73-84Informations de copyright
Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.