Characterization of Antigenic MHC-Class-I-Restricted T Cell Epitopes in the Glycoprotein of Ebolavirus.
CD8 T cells
Ebola
HLA-restriction
epitope-mapping
humans
vaccine
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
26 11 2019
26 11 2019
Historique:
received:
11
04
2019
revised:
20
07
2019
accepted:
25
10
2019
entrez:
28
11
2019
pubmed:
28
11
2019
medline:
21
10
2020
Statut:
ppublish
Résumé
Ebolavirus causes highly lethal hemorrhagic fever in humans. The envelope-displayed viral glycoprotein (GP) is the primary target of humoral immunity induced by natural exposure and vaccination. No T cell epitopes in the GP have been characterized in humans. A phase I clinical trial of a heterologous prime-boost vaccination regime with viral vectors encoding filovirus antigens elicits humoral and T cell responses in vaccinees. The most frequently recognized peptide pools are deconvoluted to identify the minimal epitopes recognized by antigen-specific T cells. We characterize nine immunogenic epitopes on the Ebolavirus GP. Histocompatibility leukocyte antigen (HLA) typing with in silico epitope analysis determines the likely MHC class I restriction elements. Thirteen HLA-A and -B alleles are predicted to present the identified CD8
Identifiants
pubmed: 31775024
pii: S2211-1247(19)31438-X
doi: 10.1016/j.celrep.2019.10.105
pmc: PMC6899439
pii:
doi:
Substances chimiques
Epitopes, T-Lymphocyte
0
Glycoproteins
0
Histocompatibility Antigens Class I
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2537-2545.e3Subventions
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : NIAID NIH HHS
ID : HHSN272200800044C
Pays : United States
Organisme : Medical Research Council
ID : MR/L009528/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/S007555/1
Pays : United Kingdom
Informations de copyright
Copyright © 2019 The Author(s). Published by Elsevier Inc. All rights reserved.
Références
Sci Transl Med. 2013 Aug 21;5(199):199ra113
pubmed: 23966302
PLoS One. 2013 Jun 21;8(6):e67016
pubmed: 23805289
Bioinformatics. 2008 Jun 1;24(11):1397-8
pubmed: 18413329
Cell. 2016 Jan 14;164(1-2):258-268
pubmed: 26771495
Curr Opin Struct Biol. 2009 Aug;19(4):408-17
pubmed: 19559599
J Infect Dis. 2017 Jan 15;215(2):287-292
pubmed: 27799354
PLoS One. 2017 Apr 27;12(4):e0176418
pubmed: 28448594
J Mol Biol. 2007 Sep 21;372(3):774-97
pubmed: 17681537
Proc Natl Acad Sci U S A. 2010 Jul 13;107(28):12599-604
pubmed: 20616031
Infect Genet Evol. 2015 Dec;36:369-375
pubmed: 26462623
J Biomed Inform. 2015 Feb;53:405-14
pubmed: 25464113
Proc Natl Acad Sci U S A. 2011 May 31;108(22):9178-83
pubmed: 21562214
J Immunol Methods. 2016 Mar;430:43-50
pubmed: 26826277
Immunogenetics. 2009 Jan;61(1):1-13
pubmed: 19002680
Nature. 2014 Oct 2;514(7520):47-53
pubmed: 25171469
JAMA. 2016 Apr 19;315(15):1610-23
pubmed: 27092831
Proc Natl Acad Sci U S A. 2015 Apr 14;112(15):4719-24
pubmed: 25775592
Science. 2014 Sep 12;345(6202):1369-72
pubmed: 25214632
Methods Mol Biol. 2018;1802:49-62
pubmed: 29858801
J Immunol. 2019 May 1;202(9):2636-2647
pubmed: 30918042
Lancet. 2017 Feb 4;389(10068):505-518
pubmed: 28017403
J Immunol Res. 2017;2017:2680160
pubmed: 29445754
N Engl J Med. 2014 Oct 9;371(15):1418-25
pubmed: 24738640
N Engl J Med. 2016 Apr 28;374(17):1647-60
pubmed: 25830326
J Immunol. 2008 Feb 15;180(4):2174-86
pubmed: 18250424
Science. 2009 Jul 10;325(5937):204-6
pubmed: 19590002
Proc Natl Acad Sci U S A. 2018 Aug 7;115(32):E7578-E7586
pubmed: 30038008
EBioMedicine. 2017 May;19:107-118
pubmed: 28434944
Tissue Antigens. 2004 Apr;63(4):293-325
pubmed: 15009803
J Infect Dis. 2019 Apr 8;219(8):1187-1197
pubmed: 30407513
Nucleic Acids Res. 2015 Jan;43(Database issue):D784-8
pubmed: 25414323
Nature. 2008 Jul 10;454(7201):177-82
pubmed: 18615077
Virology. 2007 Apr 10;360(2):257-63
pubmed: 17123567
Lancet. 2011 Mar 5;377(9768):849-62
pubmed: 21084112
N Engl J Med. 1983 Jul 7;309(1):13-7
pubmed: 6602294
Cell Rep. 2017 Aug 29;20(9):2251-2261
pubmed: 28854372
Virology. 2015 Aug;482:202-7
pubmed: 25880111
Nat Rev Microbiol. 2009 May;7(5):393-400
pubmed: 19369954
N Engl J Med. 2016 Apr 28;374(17):1635-46
pubmed: 25629663
Nat Med. 2007 Jan;13(1):100-6
pubmed: 17187074
PLoS Negl Trop Dis. 2018 May 24;12(5):e0006530
pubmed: 29795572
Virol J. 2012 Jun 13;9:111
pubmed: 22695180
Arch Virol. 2010 Dec;155(12):2083-103
pubmed: 21046175
J Virol. 2002 Jun;76(12):6408-12
pubmed: 12021376
Nat Med. 2011 Aug 21;17(9):1128-31
pubmed: 21857654
Lancet. 2015 Aug 29;386(9996):857-66
pubmed: 26248676
PLoS One. 2014 Jun 10;9(6):e96360
pubmed: 24914933
Mol Ther. 2016 Apr;24(4):832-42
pubmed: 26743582
Science. 2015 Apr 3;348(6230):117-9
pubmed: 25814067
Proc Natl Acad Sci U S A. 2014 Dec 2;111(48):17182-7
pubmed: 25404321
Front Immunol. 2019 Apr 26;10:655
pubmed: 31105686
Lancet Infect Dis. 2015 Oct;15(10):1156-1166
pubmed: 26248510
Lancet. 1990 Mar 3;335(8688):502-5
pubmed: 1968529
Nature. 2016 Jul 7;535(7610):169-172
pubmed: 27362232
Future Virol. 2009;4(6):621-635
pubmed: 20198110
Cell Res. 2009 Feb;19(2):271-3
pubmed: 19153597
Science. 2016 Mar 18;351(6279):1339-42
pubmed: 26917593
Virology. 2004 Jan 5;318(1):224-30
pubmed: 14972550
Science. 2016 Mar 18;351(6279):1343-6
pubmed: 26917592
Nature. 2016 May 5;533(7601):100-4
pubmed: 27147028
Scand J Immunol. 2016 May;83(5):321-37
pubmed: 26939891