Genomic background of a colistin-resistant and highly virulent MCR-1-positive Escherichia coli ST6395 from a broiler chicken in Pakistan.
Animals
Anti-Bacterial Agents
/ pharmacology
Chickens
/ microbiology
Colistin
/ pharmacology
Drug Resistance, Bacterial
Escherichia coli
/ drug effects
Escherichia coli Infections
/ veterinary
Escherichia coli Proteins
/ genetics
Genomics
Pakistan
Poultry Diseases
/ drug therapy
Virulence
/ genetics
Virulence Factors
Galleria mellonella
WGS
poultry
resistome; colistin resistance
virulome
Journal
Pathogens and disease
ISSN: 2049-632X
Titre abrégé: Pathog Dis
Pays: United States
ID NLM: 101595366
Informations de publication
Date de publication:
01 10 2019
01 10 2019
Historique:
received:
08
08
2019
accepted:
28
11
2019
pubmed:
30
11
2019
medline:
14
5
2020
entrez:
30
11
2019
Statut:
ppublish
Résumé
The convergence of high virulence and multidrug resistance (MDR) in Gram-negative pathogens circulating at the human-animal interface is a critical public health issue. We hereby report the genomic characteristics and virulent behavior of a colistin-resistant Escherichia coli, serotype ONT:H26, belonging to ST6395, isolated from a healthy broiler in Pakistan. This strain harbored multiple antimicrobial resistance genes, including mcr-1.1 and blaCARB-2, besides cma (colicin M) and astA [heat-stable enterotoxin 1 (EAST1) toxin] virulence genes. In vivo experiments carried out with the Galleria mellonella infection model revealed that MCR-1-positive E. coli ST6395 killed 96.4% of the larvae at 18 hour post-infection. Interplay between resistance and virulence in clinically important pathogens could be a potential threat, representing a serious challenge to global public health.
Identifiants
pubmed: 31782775
pii: 5647353
doi: 10.1093/femspd/ftz064
pii:
doi:
Substances chimiques
Anti-Bacterial Agents
0
Escherichia coli Proteins
0
MCR-1 protein, E coli
0
Virulence Factors
0
Colistin
Z67X93HJG1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© FEMS 2019.