A first approach using micellar electrokinetic capillary chromatography for the determination of fipronil and fipronil-sulfone in eggs.
Capillary electrophoresis
egg
fipronil
fipronil-sulfone
micellar electrokinetic capillary chromatography
salting-out liquid-liquid extraction
Journal
Electrophoresis
ISSN: 1522-2683
Titre abrégé: Electrophoresis
Pays: Germany
ID NLM: 8204476
Informations de publication
Date de publication:
02 2020
02 2020
Historique:
received:
02
08
2019
revised:
30
10
2019
accepted:
21
11
2019
pubmed:
1
12
2019
medline:
13
2
2021
entrez:
1
12
2019
Statut:
ppublish
Résumé
Fipronil is an insecticide that is not approved in the European Union in food. In 2017, fipronil was involved in a European health alert due to its presence in fresh hen eggs because of an illicit use in poultry farms, so reliable methods are needed to determine fipronil and its main metabolites in these matrixes. In this work, we report the first approach to the study of fipronil and two metabolites, fipronil-sulfone and fipronil-sulfide by CE. MEKC mode was employed using a solution of 50 mM ammonium perfluorooctanoate pH 9.0 with 10% (v/v) methanol as background electrolyte. The proposed method was combined with a simple sample treatment based on salting-out assisted LLE (SALLE) using acetonitrile as extraction solvent and ammonium sulfate as salt. The SALLE-MEKC-UV method allowed the simultaneous quantification of fipronil and fipronil-sulfone. Validation parameters yielded satisfactory results, with precision, expressed as relative SD, below 14% and recoveries higher than 83%. Limits of detection were 90 µg/kg for fipronil and 150 µg/kg for fipronil-sulfone, so in terms of sensitivity further studies of sample treatments allowing extra preconcentration or the use of more sensitive detection, such as MS, would be needed.
Identifiants
pubmed: 31785119
doi: 10.1002/elps.201900291
doi:
Substances chimiques
Acetonitriles
0
Insecticides
0
Pyrazoles
0
fipronil sulfone
KE7T0T1PQ7
fipronil
QGH063955F
acetonitrile
Z072SB282N
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
202-208Subventions
Organisme : European Regional Development Fund
ID : CZ.02.1.01/0.0/0.0/15_003/0000465
Pays : International
Organisme : Ministerio de Ciencia, Innovación y Universidades
ID : FPU17/03810
Pays : International
Organisme : Secretaría de Estado de Investigación, Desarrollo e Innovación
ID : AGL2015-70708-R
Pays : International
Organisme : Universidad de Granada
ID : 2013/2
Pays : International
Informations de copyright
© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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