A Simple Guide for Generating BAC Transgenic Animals for Retinal Research.

Animals Chromosomes, Artificial, Bacterial / genetics Gene Transfer Techniques Genes, Reporter / genetics Mice Mice, Inbred C57BL Mice, Transgenic / genetics Recombination, Genetic / genetics Regulatory Elements, Transcriptional / genetics Retina / physiology Transgenes / genetics
BAC sequencing BAC transgenics Colony real-time PCR Electroporation Lif Minimal media Recombineering SW102 galK

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2020
Historique:
entrez: 2 12 2019
pubmed: 2 12 2019
medline: 31 12 2020
Statut: ppublish

Résumé

Bacterial artificial chromosomes (BACs) are genomic tools that can carry several hundred kilobases of exogenous genomic material. This allows to incorporate sufficiently large DNA stretches to include most if not all upstream and downstream cis-regulatory elements of a gene in order to mimic and analyze its endogenous regulation of expression using a reporter protein in vivo. Here, we illustrate the generation of a BAC:LIF-EGFP transgenic mouse line to describe a simplified version of BAC transgenesis using galK-based recombineering.

Identifiants

DOI: 10.1007/978-1-0716-0175-4_9 PMID: 31786785
pubmed: 31786785
doi: 10.1007/978-1-0716-0175-4_9
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

109-122

Auteurs

Cavit Agca (C)

Lab for Retinal Cell Biology, Department of Ophthalmology, University of Zurich, Zurich, Switzerland. cavit.agca@sabanciuniv.edu.
Molecular Biology, Genetics and Bioengineering Program, Sabanci University, Istanbul, Turkey. cavit.agca@sabanciuniv.edu.
Nanotechnology Research and Application Center (SUNUM), Sabanci University, Istanbul, Turkey. cavit.agca@sabanciuniv.edu.

Christian Grimm (C)

Lab for Retinal Cell Biology, Department of Ophthalmology, University of Zurich, Zurich, Switzerland.
Zurich Center for Integrative Human Physiology (ZIHP), University of Zurich, Zurich, Switzerland.
Neuroscience Center (ZNZ), University of Zurich, Zurich, Switzerland.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › A Simple Guide for Generating BAC Transgenic...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Vecteurs génétiques › Chromosomes artificiels › Chromosomes artificiels de bactérie › A Simple Guide for Generating BAC Transgenic...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques de transfert de gènes › A Simple Guide for Generating BAC Transgenic...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Gènes › Gènes rapporteurs › A Simple Guide for Generating BAC Transgenic...
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