Single-Tube Multiplex Polymerase Chain Reaction for the Detection of Genes Encoding Enterobacteriaceae Carbapenemase.

Bacterial Proteins / genetics DNA Primers / genetics Enterobacteriaceae / enzymology Enterobacteriaceae Infections / diagnosis Genes, Bacterial Humans Multiplex Polymerase Chain Reaction / methods Sensitivity and Specificity beta-Lactamases / genetics
Carbanem resistant Enterobacteriaceae Enterobacteriaceae carbapenemase genes multiplex PCR

Journal

Japanese journal of infectious diseases
ISSN: 1884-2836
Titre abrégé: Jpn J Infect Dis
Pays: Japan
ID NLM: 100893704

Informations de publication

Date de publication:
24 Mar 2020
Historique:
pubmed: 4 12 2019
medline: 21 10 2020
entrez: 3 12 2019
Statut: ppublish

Résumé

A multiplex PCR assay in a single tube was developed for the detection of the carbapenemase genes of Enterobacteriaceae. Primers were designed to amplify the following six carbapenemase genes: bla

Identifiants

DOI: 10.7883/yoken.JJID.2019.041 PMID: 31787735
pubmed: 31787735
doi: 10.7883/yoken.JJID.2019.041
doi:

Substances chimiques

Bacterial Proteins 0
DNA Primers 0
beta-Lactamases EC 3.5.2.6
carbapenemase EC 3.5.2.6

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

166-172

Auteurs

Masanori Watahiki (M)

Department of Bacteriology, Toyama Institute of Health.

Ryuji Kawahara (R)

Division of Microbiology, Osaka Institute of Public Health.

Masahiro Suzuki (M)

Department of Microbiology and Medical Zoology, Aichi Prefectural Institute of Public Health.
Present Address: Department of Microbiology, School of Medicine, Fujita Health University.

Miyako Aoki (M)

Department of Microbiology and Medical Zoology, Aichi Prefectural Institute of Public Health.

Kaoru Uchida (K)

Department of Bacteriology, Toyama Institute of Health.

Yuko Matsumoto (Y)

Microbiological Testing and Research Division, Yokohama City Institute of Public Health.

Yuko Kumagai (Y)

Hygiene Division, Bacteriology Section, Akita Prefectural Research Center for Public Health and Environment.

Makiko Noda (M)

Department of Infectious Diseases, Gifu Prefectural Research Institute for Health and Environmental Sciences.

Kanako Masuda (K)

Hiroshima Prefectural Technology Research Institute, Public Health and Environment Center.

Chiemi Fukuda (C)

Department of Microbiology, Kagawa Prefectural Research Institute for Environmental Sciences and Public Health.

Seiya Harada (S)

Department of Microbiology, Kumamoto Prefectural Institute of Public Health and Environmental Science.

Keiko Senba (K)

Department of Microbiology, Ehime Prefectural Institute of Public Health and Environmental Science.

Masato Suzuki (M)

Antimicrobial Resistance Research Center, National Institute of Infectious Diseases.

Mari Matsui (M)

Antimicrobial Resistance Research Center, National Institute of Infectious Diseases.

Satowa Suzuki (S)

Antimicrobial Resistance Research Center, National Institute of Infectious Diseases.

Keigo Shibayama (K)

Department of Bacteriology II, National Institute of Infectious Diseases.

Hiroto Shinomiya (H)

Department of Microbiology, Ehime Prefectural Institute of Public Health and Environmental Science.

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Single-Tube Multiplex Polymerase Chain...
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Produits chimiques de laboratoire › Sondes moléculaires › Sondes d'acide nucléique › Sondes d'ADN › Amorces ADN › Single-Tube Multiplex Polymerase Chain...
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Enterobacteriaceae › Single-Tube Multiplex Polymerase Chain...
questionsmedicales.fr › Infection › Infections bactériennes et mycoses › Infections bactériennes › Infections bactériennes à Gram négatif › Infections à Enterobacteriaceae › Single-Tube Multiplex Polymerase Chain...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Génome microbien › Génome bactérien › Gènes bactériens › Single-Tube Multiplex Polymerase Chain...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Single-Tube Multiplex Polymerase Chain...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › Réaction de polymérisation en chaîne › Réaction de polymérisation en chaine multiplex › Single-Tube Multiplex Polymerase Chain...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Statistiques comme sujet › Sensibilité et spécificité › Single-Tube Multiplex Polymerase Chain...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Amidohydrolases › bêta-Lactamases › Single-Tube Multiplex Polymerase Chain...