Evaluation of the Unyvero i60 ITI® multiplex PCR for infected chronic leg ulcers diagnosis.
Infected chronic leg ulcer
Microbiological diagnosis
Multiplex PCR
Unyvero i60 ITI system
Journal
Journal of microbiological methods
ISSN: 1872-8359
Titre abrégé: J Microbiol Methods
Pays: Netherlands
ID NLM: 8306883
Informations de publication
Date de publication:
01 2020
01 2020
Historique:
received:
16
05
2019
revised:
27
11
2019
accepted:
28
11
2019
pubmed:
4
12
2019
medline:
13
2
2021
entrez:
3
12
2019
Statut:
ppublish
Résumé
Unyvero i60 ITI multiplex PCR (mPCR) may identify a large panel of bacteria and antibiotic resistance genes. In this study, we compared results obtained by mPCR to standard bacteriology in chronic leg ulcer (CLU) infections. A prospective study, part of the interventional-blinded randomized study "ulcerinfecte" (NCT02889926), was conducted at Saint Joseph Hospital in Paris. Fifty patients with a suspicion of infected CLU were included between February 2017 and September 2018. Conventional bacteriology and mPCR were performed simultaneously on deep skin biopsies. Staphylococcus aureus and Pseudomonas aeruginosa were the most detected pathogens. Regarding the global sensitivity, mPCR is not overcome to the standard culture. Anaerobes and slow growing bacteria were detected with a higher sensitivity rate by mPCR than standard culture. Unyvero i60 ITI multiplex PCR detected rapidly pathogenic bacteria in infected CLU especially anaerobes and slow growing bacteria and was particularly effective for patients previously treated with antibiotics.
Identifiants
pubmed: 31790779
pii: S0167-7012(19)30364-1
doi: 10.1016/j.mimet.2019.105796
pii:
doi:
Types de publication
Comparative Study
Journal Article
Randomized Controlled Trial
Langues
eng
Sous-ensembles de citation
IM
Pagination
105796Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.