The Oncogenic Kaposi's Sarcoma-Associated Herpesvirus Encodes a Mimic of the Tumor-Suppressive miR-15/16 miRNA Family.


Journal

Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691

Informations de publication

Date de publication:
03 12 2019
Historique:
received: 10 06 2019
revised: 07 10 2019
accepted: 01 11 2019
entrez: 5 12 2019
pubmed: 5 12 2019
medline: 29 9 2020
Statut: ppublish

Résumé

Many tumor viruses encode oncogenes of cellular origin. Here, we report an oncoviral mimic of a cellular tumor suppressor. The Kaposi's sarcoma-associated herpesvirus (KSHV) microRNA (miRNA) miR-K6-5p shares sequence similarity to the tumor-suppressive cellular miR-15/16 miRNA family. We show that miR-K6-5p inhibits cell cycle progression, a hallmark function of miR-16. miR-K6-5p regulates conserved miR-15/16 family miRNA targets, including many cell cycle regulators. Inhibition of miR-K6-5p in KSHV-transformed B cells confers a significant growth advantage. Altogether, our data show that KSHV encodes a functional mimic of miR-15/16 family miRNAs. While it is exceedingly well established that oncogenic viruses encode oncogenes of cellular origin, this is an unusual example of an oncogenic virus that encodes a viral mimic of a cellular tumor suppressor. Encoding a tumor-suppressive miRNA could help KSHV balance viral oncogene expression and thereby avoid severe pathogenesis in the healthy host.

Identifiants

pubmed: 31801064
pii: S2211-1247(19)31476-7
doi: 10.1016/j.celrep.2019.11.005
pmc: PMC6939447
mid: NIHMS1545828
pii:
doi:

Substances chimiques

MIRN15 microRNA, human 0
MIRN16 microRNA, human 0
MicroRNAs 0
RNA, Viral 0

Types de publication

Journal Article Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

2961-2969.e6

Subventions

Organisme : NCI NIH HHS
ID : R01 CA180813
Pays : United States
Organisme : NCI NIH HHS
ID : R21 CA210904
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM008061
Pays : United States

Informations de copyright

Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.

Références

RNA. 2014 Jun;20(6):923-37
pubmed: 24757167
Blood. 1995 Aug 15;86(4):1276-80
pubmed: 7632932
Blood. 2005 Mar 15;105(6):2510-8
pubmed: 15572586
J Virol. 2013 Nov;87(21):11908-11
pubmed: 23966392
J Pathol. 2008 May;215(1):13-20
pubmed: 18348159
PLoS Pathog. 2017 Jan 19;13(1):e1006078
pubmed: 28103305
Proc Natl Acad Sci U S A. 2008 Apr 1;105(13):5166-71
pubmed: 18362358
Blood. 2009 May 7;113(19):4586-94
pubmed: 19202128
Bioinformatics. 2015 Jan 15;31(2):166-9
pubmed: 25260700
Cancer Biol Ther. 2008 Nov;7(11):1758-64
pubmed: 18708755
Proc Natl Acad Sci U S A. 2015 Sep 15;112(37):11636-41
pubmed: 26324892
Proc Natl Acad Sci U S A. 2005 Sep 27;102(39):13944-9
pubmed: 16166262
Nat Genet. 2009 Jan;41(1):130-4
pubmed: 19098914
J Virol. 2007 Dec;81(23):12836-45
pubmed: 17881434
Mol Cell. 2011 Sep 16;43(6):993-1004
pubmed: 21925387
Blood. 1996 Jul 15;88(2):645-56
pubmed: 8695812
PLoS Pathog. 2018 Dec 17;14(12):e1007441
pubmed: 30557332
Nat Med. 1995 Dec;1(12):1274-8
pubmed: 7489408
Genome Res. 2009 Jan;19(1):92-105
pubmed: 18955434
J Virol. 2018 Mar 28;92(8):
pubmed: 29386283
Aging (Albany NY). 2018 Aug 09;10(8):1884-1901
pubmed: 30103211
Nat Protoc. 2009;4(1):44-57
pubmed: 19131956
Cell. 2013 Apr 25;153(3):654-65
pubmed: 23622248
Blood. 2004 Nov 15;104(10):3349-54
pubmed: 15271792
J Virol. 2010 May;84(10):5229-37
pubmed: 20219912
Genome Biol. 2014;15(12):550
pubmed: 25516281
J Virol. 2011 Oct;85(19):9877-86
pubmed: 21813606
Mol Cell Biol. 2007 Mar;27(6):2240-52
pubmed: 17242205
RNA. 2015 Sep;21(9):1606-20
pubmed: 26137849
Proc Natl Acad Sci U S A. 2018 Dec 18;115(51):13069-13074
pubmed: 30478046
Methods Enzymol. 2011;498:349-61
pubmed: 21601685
J Clin Invest. 2012 Apr;122(4):1487-502
pubmed: 22406538
Mol Cell. 2007 Jun 8;26(5):745-52
pubmed: 17540599
Sci Rep. 2017 Jan 19;7:40813
pubmed: 28102325
Nat Commun. 2018 Aug 15;9(1):3263
pubmed: 30111820
Cell Host Microbe. 2012 Feb 16;11(2):167-80
pubmed: 22341465
J Virol. 2013 Nov;87(21):11821-30
pubmed: 23986579
Cell Host Microbe. 2011 Nov 17;10(5):515-26
pubmed: 22100165
Cell. 2017 Feb 23;168(5):890-903.e15
pubmed: 28162770
PLoS Pathog. 2013;9(9):e1003584
pubmed: 24039573
EMBO J. 2009 Jul 22;28(14):2090-9
pubmed: 19536137
Nature. 2007 Dec 13;450(7172):1096-9
pubmed: 18075594
Cell. 2018 Mar 22;173(1):20-51
pubmed: 29570994
PLoS One. 2012;7(11):e49435
pubmed: 23185331
N Engl J Med. 1995 May 4;332(18):1186-91
pubmed: 7700311
Blood. 2008 Aug 1;112(3):822-9
pubmed: 18483394
J Virol. 2017 Jan 18;91(3):
pubmed: 27852859
Proc Natl Acad Sci U S A. 2005 Oct 25;102(43):15545-50
pubmed: 16199517
Cell Physiol Biochem. 2018;48(2):670-682
pubmed: 30025387
Cell. 2009 Jan 23;136(2):215-33
pubmed: 19167326
Cancer Cell. 2010 Jan 19;17(1):28-40
pubmed: 20060366
Bioinformatics. 2013 Jan 1;29(1):15-21
pubmed: 23104886
Mol Cell. 2014 Jun 19;54(6):1042-1054
pubmed: 24857550
J Virol. 2010 Nov;84(22):11670-8
pubmed: 20844043
Cell. 2015 Dec 3;163(6):1515-26
pubmed: 26627737
Proc Natl Acad Sci U S A. 2002 Nov 26;99(24):15524-9
pubmed: 12434020
Nucleic Acids Res. 2008 Sep;36(16):5391-404
pubmed: 18701644
Elife. 2015 Aug 12;4:null
pubmed: 26267216
J Exp Med. 2004 Apr 5;199(7):993-1003
pubmed: 15067035
Silence. 2011 Jun 07;2:4
pubmed: 21649885
Science. 2014 Jan 3;343(6166):80-4
pubmed: 24336569
Blood. 2008 Jan 1;111(1):320-7
pubmed: 17890449
PLoS Pathog. 2012;8(8):e1002884
pubmed: 22927820
Blood. 2007 Jun 1;109(11):4944-51
pubmed: 17327404
Nat Methods. 2007 Sep;4(9):721-6
pubmed: 17694064
Science. 1994 Dec 16;266(5192):1865-9
pubmed: 7997879

Auteurs

Kylee Morrison (K)

Department of Microbiology-Immunology, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA.

Mark Manzano (M)

Department of Microbiology-Immunology, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA.

Kevin Chung (K)

Department of Microbiology-Immunology, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA.

Matthew J Schipma (MJ)

Department of Biochemistry and Molecular Genetics, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA.

Elizabeth T Bartom (ET)

Department of Biochemistry and Molecular Genetics, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA.

Eva Gottwein (E)

Department of Microbiology-Immunology, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA. Electronic address: e-gottwein@northwestern.edu.

Articles similaires

[Redispensing of expensive oral anticancer medicines: a practical application].

Lisanne N van Merendonk, Kübra Akgöl, Bastiaan Nuijen
1.00
Humans Antineoplastic Agents Administration, Oral Drug Costs Counterfeit Drugs

Smoking Cessation and Incident Cardiovascular Disease.

Jun Hwan Cho, Seung Yong Shin, Hoseob Kim et al.
1.00
Humans Male Smoking Cessation Cardiovascular Diseases Female
Humans United States Aged Cross-Sectional Studies Medicare Part C
1.00
Humans Yoga Low Back Pain Female Male

Classifications MeSH