Spatial proximity between T and PD-L1 expressing cells as a prognostic biomarker for oropharyngeal squamous cell carcinoma.
B7-H1 Antigen
/ immunology
Biomarkers, Tumor
/ immunology
CD8-Positive T-Lymphocytes
/ immunology
Deep Learning
Humans
Lymphocytes, Tumor-Infiltrating
/ immunology
Oropharyngeal Neoplasms
/ immunology
Prognosis
Squamous Cell Carcinoma of Head and Neck
/ immunology
Tumor Escape
/ immunology
Tumor Microenvironment
/ immunology
Journal
British journal of cancer
ISSN: 1532-1827
Titre abrégé: Br J Cancer
Pays: England
ID NLM: 0370635
Informations de publication
Date de publication:
02 2020
02 2020
Historique:
received:
21
05
2019
accepted:
21
10
2019
revised:
15
10
2019
pubmed:
7
12
2019
medline:
22
10
2020
entrez:
7
12
2019
Statut:
ppublish
Résumé
Fulfilling the promise of cancer immunotherapy requires novel predictive biomarkers to characterise the host immune microenvironment. Deciphering the complexity of immune cell interactions requires an automated multiplex approach to histological analysis of tumour sections. We tested a new automatic approach to select tissue and quantify the frequencies of cell-cell spatial interactions occurring in the PD1/PD-L1 pathway, hypothesised to reflect immune escape in oropharyngeal squamous cell carcinoma (OPSCC). Single sections of diagnostic biopsies from 72 OPSCC patients were stained using multiplex immunofluorescence (CD8, PD1, PD-L1, CD68). Following multispectral scanning and automated regions-of-interest selection, the Hypothesised Interaction Distribution (HID) method quantified spatial proximity between cells. Method applicability was tested by investigating the prognostic significance of co-localised cells (within 30 μm) in patients stratified by HPV status. High frequencies of proximal CD8 The HID method can quantify spatial interactions considered to reflect immune escape and generate prognostic information in OPSCC. The new automated approach is ready to test in additional cohorts and its applicability should be explored in research and clinical studies.
Sections du résumé
BACKGROUND
Fulfilling the promise of cancer immunotherapy requires novel predictive biomarkers to characterise the host immune microenvironment. Deciphering the complexity of immune cell interactions requires an automated multiplex approach to histological analysis of tumour sections. We tested a new automatic approach to select tissue and quantify the frequencies of cell-cell spatial interactions occurring in the PD1/PD-L1 pathway, hypothesised to reflect immune escape in oropharyngeal squamous cell carcinoma (OPSCC).
METHODS
Single sections of diagnostic biopsies from 72 OPSCC patients were stained using multiplex immunofluorescence (CD8, PD1, PD-L1, CD68). Following multispectral scanning and automated regions-of-interest selection, the Hypothesised Interaction Distribution (HID) method quantified spatial proximity between cells. Method applicability was tested by investigating the prognostic significance of co-localised cells (within 30 μm) in patients stratified by HPV status.
RESULTS
High frequencies of proximal CD8
CONCLUSION
The HID method can quantify spatial interactions considered to reflect immune escape and generate prognostic information in OPSCC. The new automated approach is ready to test in additional cohorts and its applicability should be explored in research and clinical studies.
Identifiants
pubmed: 31806878
doi: 10.1038/s41416-019-0634-z
pii: 10.1038/s41416-019-0634-z
pmc: PMC7028988
doi:
Substances chimiques
B7-H1 Antigen
0
Biomarkers, Tumor
0
CD274 protein, human
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
539-544Subventions
Organisme : Cancer Research UK (CRUK)
ID : C147/A25254
Pays : International
Organisme : Cancer Research UK (CRUK)
ID : C147/A2525
Pays : International
Organisme : Cancer Research UK (CRUK)
ID : C147/A2525
Pays : International
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