Convergence between Microglia and Peripheral Macrophages Phenotype during Development and Neuroinflammation.
Animals
Brain
/ cytology
Cell Differentiation
/ physiology
Cell Plasticity
/ physiology
Inflammation
/ metabolism
Macrophages
/ cytology
Membrane Glycoproteins
/ genetics
Membrane Proteins
/ genetics
Mice
Microglia
/ cytology
Phenotype
Receptors, Immunologic
/ genetics
Receptors, Purinergic P2Y12
/ genetics
Transcriptome
EAE
development
macrophages
microglia
plasticity
tissue-reprogramming
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
ISSN: 1529-2401
Titre abrégé: J Neurosci
Pays: United States
ID NLM: 8102140
Informations de publication
Date de publication:
22 01 2020
22 01 2020
Historique:
received:
25
06
2019
revised:
18
10
2019
accepted:
23
10
2019
pubmed:
11
12
2019
medline:
24
7
2020
entrez:
11
12
2019
Statut:
ppublish
Résumé
Differently from other myeloid cells, microglia derive exclusively from precursors originating within the yolk sac and migrate to the CNS under development, without any contribution from fetal liver or postnatal hematopoiesis. Consistent with their unique ontology, microglia may express specific physiological markers, which have been partly described in recent years. Here we wondered whether profiles distinguishing microglia from peripheral macrophages vary with age and under pathology. To this goal, we profiled transcriptomes of microglia throughout the lifespan and included a parallel comparison with peripheral macrophages under physiological and neuroinflammatory settings using age- and sex-matched wild-type and bone marrow chimera mouse models. This comprehensive approach demonstrated that the phenotypic differentiation between microglia and peripheral macrophages is age-dependent and that peripheral macrophages do express some of the most commonly described microglia-specific markers early during development, such as Fcrls, P2ry12, Tmem119, and Trem2. Further, during chronic neuroinflammation CNS-infiltrating macrophages and not peripheral myeloid cells acquire microglial markers, indicating that the CNS niche may instruct peripheral myeloid cells to gain the phenotype and, presumably, the function of the microglia cell. In conclusion, our data provide further evidence about the plasticity of the myeloid cell and suggest caution in the strict definition and application of microglia-specific markers.
Identifiants
pubmed: 31818979
pii: JNEUROSCI.1523-19.2019
doi: 10.1523/JNEUROSCI.1523-19.2019
pmc: PMC6975289
doi:
Substances chimiques
Membrane Glycoproteins
0
Membrane Proteins
0
Obif protein, mouse
0
P2ry12 protein, mouse
0
Receptors, Immunologic
0
Receptors, Purinergic P2Y12
0
Trem2 protein, mouse
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
784-795Informations de copyright
Copyright © 2020 the authors.
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