Cdc7 kinase stimulates Aurora B kinase in M-phase.

Animals Aurora Kinase B / metabolism Cell Cycle Cell Cycle Proteins / metabolism Cell Division Cell Line, Tumor Centromere / metabolism Chromosomal Proteins, Non-Histone / metabolism HCT116 Cells HEK293 Cells HeLa Cells Humans Insecta Mitosis Mutation Phosphorylation Protein Serine-Threonine Kinases / metabolism Rats Spindle Apparatus / metabolism Survivin / metabolism Threonine / chemistry

Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
09 12 2019
Historique:
received: 27 09 2017
accepted: 16 08 2019
entrez: 11 12 2019
pubmed: 11 12 2019
medline: 16 12 2020
Statut: epublish

Résumé

The conserved serine-threonine kinase, Cdc7, plays a crucial role in initiation of DNA replication by facilitating the assembly of an initiation complex. Cdc7 is expressed at a high level and exhibits significant kinase activity not only during S-phase but also during G2/M-phases. A conserved mitotic kinase, Aurora B, is activated during M-phase by association with INCENP, forming the chromosome passenger complex with Borealin and Survivin. We show that Cdc7 phosphorylates and stimulates Aurora B kinase activity in vitro. We identified threonine-236 as a critical phosphorylation site on Aurora B that could be a target of Cdc7 or could be an autophosphorylation site stimulated by Cdc7-mediated phosphorylation elsewhere. We found that threonines at both 232 (that has been identified as an autophosphorylation site) and 236 are essential for the kinase activity of Aurora B. Cdc7 down regulation or inhibition reduced Aurora B activity in vivo and led to retarded M-phase progression. SAC imposed by paclitaxel was dramatically reversed by Cdc7 inhibition, similar to the effect of Aurora B inhibition under the similar situation. Our data show that Cdc7 contributes to M-phase progression and to spindle assembly checkpoint most likely through Aurora B activation.

Identifiants

DOI: 10.1038/s41598-019-54738-2 PMID: 31819079 PMC: PMC6901529
pubmed: 31819079
doi: 10.1038/s41598-019-54738-2
pii: 10.1038/s41598-019-54738-2
pmc: PMC6901529
doi:

Substances chimiques

Birc5 protein, rat 0
Cell Cycle Proteins 0
Chromosomal Proteins, Non-Histone 0
Survivin 0
Threonine 2ZD004190S
CDC7 protein, human EC 2.7.1.-
AURKB protein, human EC 2.7.11.1
Aurkb protein, rat EC 2.7.11.1
Aurora Kinase B EC 2.7.11.1
Protein Serine-Threonine Kinases EC 2.7.11.1

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

18622

Références

Sci Rep. 2015 Jul 24;5:12204
pubmed: 26206521
Cell Cycle. 2010 Dec 1;9(23):4627-37
pubmed: 21099360
Mol Cell Biol. 2000 May;20(10):3667-76
pubmed: 10779356
Mol Cell. 2005 Apr 29;18(3):379-91
pubmed: 15866179
Proc Natl Acad Sci U S A. 2006 Aug 1;103(31):11521-6
pubmed: 16864800
Genes Dev. 1997 Dec 15;11(24):3365-74
pubmed: 9407029
J Cell Biol. 2001 Dec 24;155(7):1147-57
pubmed: 11756469
Nat Cell Biol. 2010 Mar;12(3):294-8
pubmed: 20139971
Mol Cell Biol. 1999 Dec;19(12):8625-32
pubmed: 10567586
J Biol Chem. 2000 Sep 15;275(37):29042-52
pubmed: 10846177
Nat Cell Biol. 2009 Mar;11(3):357-62
pubmed: 19182789
Nature. 1998 Dec 10;396(6711):580-4
pubmed: 9859993
Mol Cell. 2008 Aug 8;31(3):438-48
pubmed: 18691976
Nature. 2010 Oct 7;467(7316):719-23
pubmed: 20739936
J Mol Cell Biol. 2011 Aug;3(4):260-7
pubmed: 21148584
J Cell Biol. 2011 Oct 31;195(3):449-66
pubmed: 22024163
J Cell Sci. 2019 Jan 25;132(2):
pubmed: 30635443
Mol Microbiol. 1994 Mar;11(5):805-10
pubmed: 8022258
Mol Biol Cell. 2003 Aug;14(8):3325-41
pubmed: 12925766
Genes Dev. 2010 Jun 15;24(12):1208-19
pubmed: 20551170
Nat Commun. 2019 Feb 8;10(1):682
pubmed: 30737408
J Cell Biol. 2003 Apr 28;161(2):267-80
pubmed: 12719470
Cell Cycle. 2012 Apr 15;11(8):1490-5
pubmed: 22433949
Genes Cells. 2003 May;8(5):451-63
pubmed: 12694534
Trends Cell Biol. 2011 Mar;21(3):133-40
pubmed: 21106376
J Cell Biol. 2014 Oct 27;207(2):201-11
pubmed: 25332165
Biochem Soc Trans. 2013 Dec;41(6):1712-9
pubmed: 24256280
Nat Commun. 2016 Jul 12;7:12135
pubmed: 27401717
Mol Cell. 2006 Oct 6;24(1):101-13
pubmed: 17018296
EMBO J. 1998 Feb 2;17(3):667-76
pubmed: 9450992
Nat Rev Mol Cell Biol. 2012 Dec;13(12):789-803
pubmed: 23175282
J Med Chem. 2012 Sep 13;55(17):7841-8
pubmed: 22920039
Nature. 2015 Mar 26;519(7544):431-5
pubmed: 25739503
J Cell Biol. 2004 Jul 19;166(2):179-91
pubmed: 15249581
Annu Rev Genet. 2016 Nov 23;50:155-173
pubmed: 27617969
J Biol Chem. 2008 Jul 11;283(28):19211-8
pubmed: 18442972
Nat Rev Mol Cell Biol. 2014 Jul;15(7):433-52
pubmed: 24954208
J Biol Chem. 2004 Mar 26;279(13):12997-3003
pubmed: 14722118
EMBO J. 2016 May 2;35(9):961-73
pubmed: 26912723
J Biol Chem. 2002 Aug 2;277(31):27577-80
pubmed: 12048181
EMBO J. 2010 Sep 1;29(17):2953-65
pubmed: 20664522
Nat Struct Mol Biol. 2012 Nov;19(11):1101-7
pubmed: 23064647
Acta Crystallogr F Struct Biol Commun. 2014 Mar;70(Pt 3):294-8
pubmed: 24598913
Cell Cycle. 2013 May 15;12(10):1560-8
pubmed: 23598722
Nat Cell Biol. 2006 Feb;8(2):180-7
pubmed: 16378098
Mol Cell. 2012 Sep 14;47(5):722-33
pubmed: 22841486
Nat Cell Biol. 2003 Dec;5(12):1111-6
pubmed: 14625560
J Cell Sci. 2000 Jun;113 ( Pt 12):2111-7
pubmed: 10825284
Curr Biol. 2000 Sep 7;10(17):1075-8
pubmed: 10996078
PLoS Biol. 2012 Jan;10(1):e1001250
pubmed: 22291575
J Biol Chem. 2005 Apr 1;280(13):13062-70
pubmed: 15668232
Proc Natl Acad Sci U S A. 2006 Jul 11;103(28):10660-5
pubmed: 16818887
Methods. 2012 Jun;57(2):214-21
pubmed: 22800621
Curr Opin Cell Biol. 2009 Dec;21(6):796-805
pubmed: 19836940
Curr Biol. 2001 Jun 5;11(11):886-90
pubmed: 11516652
Genes Cells. 2002 Jan;7(1):11-7
pubmed: 11856369
Dev Cell. 2003 Dec;5(6):853-64
pubmed: 14667408
J Cell Biol. 2017 Apr 3;216(4):925-941
pubmed: 28314740
J Cell Physiol. 2002 Mar;190(3):287-96
pubmed: 11857444
Cell Rep. 2016 Apr 5;15(1):210-218
pubmed: 27052166
Genes Dev. 2013 Nov 15;27(22):2459-72
pubmed: 24240236
J Biol Chem. 2006 Dec 22;281(51):39249-61
pubmed: 17046832
PLoS One. 2012;7(5):e36372
pubmed: 22574151
J Biol Chem. 2006 Apr 28;281(17):12041-9
pubmed: 16492669
J Cell Biol. 2003 Apr 28;161(2):281-94
pubmed: 12707311
J Biol Chem. 2007 Oct 12;282(41):30029-38
pubmed: 17711849
EMBO J. 1997 Jul 16;16(14):4340-51
pubmed: 9250678
J Biol Chem. 2011 Jul 1;286(26):23031-43
pubmed: 21536671
Genes Dev. 2008 Feb 1;22(3):386-97
pubmed: 18245450
Genes Dev. 2008 Feb 1;22(3):398-410
pubmed: 18245451
Oncogene. 2008 May 29;27(24):3475-82
pubmed: 18084324
Annu Rev Biochem. 2010;79:89-130
pubmed: 20373915
Chromosoma. 1991 Mar;100(3):139-46
pubmed: 2040201
Mol Gen Genet. 1997 May 20;254(5):562-70
pubmed: 9197416

Auteurs

Sayuri Ito (S)

Department of Genome Medicine, Tokyo Metropolitan Institute of Medical Science, Tokyo, 156-8506, Japan.

Hidemasa Goto (H)

Department of Neural Regeneration and Cell Communication, Mie University Graduate School of Medicine, Tsu, Mie, 514-8507, Japan.

Kinue Kuniyasu (K)

Department of Molecular Oncology, Institute of Development, Aging and Cancer, Tohoku University, Sendai, 980-8575, Japan.

Mayumi Shindo (M)

Laboratory of Protein Analyses, Tokyo Metropolitan Institute of Medical Science, Tokyo, 156-8506, Japan.

Masayuki Yamada (M)

Medical Education Center, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University, 77900, Olomouc, Czech Republic.

Kozo Tanaka (K)

Department of Molecular Oncology, Institute of Development, Aging and Cancer, Tohoku University, Sendai, 980-8575, Japan.

Gaik-Theng Toh (GT)

Department of Genome Medicine, Tokyo Metropolitan Institute of Medical Science, Tokyo, 156-8506, Japan.

Masaaki Sawa (M)

Carna Biosciences, Inc., Kobe, Japan.
ORCID: 0000-0002-0077-2188

Masaki Inagaki (M)

Department of Physiology, Mie University Graduate School of Medicine, Tsu, Mie, 514-8507, Japan.

Jiri Bartek (J)

Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University, 77900, Olomouc, Czech Republic.
Danish Cancer Society Research Center, Copenhagen, Denmark.
Division of Genome Biology, Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden.

Hisao Masai (H)

Department of Genome Medicine, Tokyo Metropolitan Institute of Medical Science, Tokyo, 156-8506, Japan. masai-hs@igakuken.or.jp.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Cdc7 kinase stimulates Aurora B kinase in M-phase.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Protein-Serine-Threonine Kinases › Aurora kinases › Aurora kinase B › Cdc7 kinase stimulates Aurora B kinase in M-phase.
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Cycle cellulaire › Cdc7 kinase stimulates Aurora B kinase in M-phase.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines du cycle cellulaire › Cdc7 kinase stimulates Aurora B kinase in M-phase.
questionsmedicales.fr › Phénomènes physiologiques › Croissance et développement › Croissance › Processus de croissance cellulaire › Prolifération cellulaire › Division cellulaire › Cdc7 kinase stimulates Aurora B kinase in M-phase.
questionsmedicales.fr › Cellules › Cellules cultivées › Cellules cancéreuses en culture › Lignée cellulaire tumorale › Cdc7 kinase stimulates Aurora B kinase in M-phase.
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Structures de chromosome › Centromère › Cdc7 kinase stimulates Aurora B kinase in M-phase.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Nucléoprotéines › Protéines chromosomiques nonhistones › Cdc7 kinase stimulates Aurora B kinase in M-phase.
questionsmedicales.fr › Cellules › Cellules cultivées › Cellules cancéreuses en culture › Lignée cellulaire tumorale › Cellules HCT116 › Cdc7 kinase stimulates Aurora B kinase in M-phase.
questionsmedicales.fr › Cellules › Cellules épithéliales › Cellules HEK293 › Cdc7 kinase stimulates Aurora B kinase in M-phase.
questionsmedicales.fr › Cellules › Cellules épithéliales › Cellules HeLa › Cdc7 kinase stimulates Aurora B kinase in M-phase.
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Cdc7 kinase stimulates Aurora B kinase in M-phase.
questionsmedicales.fr › Eucaryotes › Animaux › Invertébrés › Arthropodes › Insectes › Cdc7 kinase stimulates Aurora B kinase in M-phase.
questionsmedicales.fr › Phénomènes génétiques › Division du noyau cellulaire › Mitose › Cdc7 kinase stimulates Aurora B kinase in M-phase.
questionsmedicales.fr › Phénomènes génétiques › Variation génétique › Mutation › Cdc7 kinase stimulates Aurora B kinase in M-phase.
questionsmedicales.fr › Métabolisme › Phosphorylation › Cdc7 kinase stimulates Aurora B kinase in M-phase.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Protein-Serine-Threonine Kinases › Cdc7 kinase stimulates Aurora B kinase in M-phase.
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Rats › Cdc7 kinase stimulates Aurora B kinase in M-phase.
questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Cytosquelette › Appareil du fuseau › Cdc7 kinase stimulates Aurora B kinase in M-phase.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Protéines régulatrices de l'apoptose › Protéines IAP › Survivine › Cdc7 kinase stimulates Aurora B kinase in M-phase.
questionsmedicales.fr › Acides aminés, peptides et protéines › Acides aminés › Acides aminés neutres › Thréonine › Cdc7 kinase stimulates Aurora B kinase in M-phase.