Nano-encapsulated Escherichia coli Divisome Anchor ZipA, and in Complex with FtsZ.
Bacterial Proteins
/ metabolism
Carrier Proteins
/ metabolism
Cell Cycle Proteins
/ metabolism
Cell Division
/ physiology
Cryoelectron Microscopy
/ methods
Cytoskeletal Proteins
/ metabolism
Escherichia coli
/ metabolism
Escherichia coli Proteins
/ metabolism
Membrane Proteins
/ metabolism
Protein Binding
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
10 12 2019
10 12 2019
Historique:
received:
13
12
2018
accepted:
10
11
2019
entrez:
12
12
2019
pubmed:
12
12
2019
medline:
24
11
2020
Statut:
epublish
Résumé
The E. coli membrane protein ZipA, binds to the tubulin homologue FtsZ, in the early stage of cell division. We isolated ZipA in a Styrene Maleic Acid lipid particle (SMALP) preserving its position and integrity with native E. coli membrane lipids. Direct binding of ZipA to FtsZ is demonstrated, including FtsZ fibre bundles decorated with ZipA. Using Cryo-Electron Microscopy, small-angle X-ray and neutron scattering, we determine the encapsulated-ZipA structure in isolation, and in complex with FtsZ to a resolution of 1.6 nm. Three regions can be identified from the structure which correspond to, SMALP encapsulated membrane and ZipA transmembrane helix, a separate short compact tether, and ZipA globular head which binds FtsZ. The complex extends 12 nm from the membrane in a compact structure, supported by mesoscale modelling techniques, measuring the movement and stiffness of the regions within ZipA provides molecular scale analysis and visualisation of the early divisome.
Identifiants
pubmed: 31822696
doi: 10.1038/s41598-019-54999-x
pii: 10.1038/s41598-019-54999-x
pmc: PMC6904479
doi:
Substances chimiques
Bacterial Proteins
0
Carrier Proteins
0
Cell Cycle Proteins
0
Cytoskeletal Proteins
0
Escherichia coli Proteins
0
FtsZ protein, Bacteria
0
Membrane Proteins
0
ZipA protein, E coli
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
18712Subventions
Organisme : RCUK | Biotechnology and Biological Sciences Research Council (BBSRC)
ID : BB/J017310/1
Pays : International
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