Chitosan, hydroxypropyltrimethyl ammonium chloride chitosan and sulfated chitosan nanoparticles as adjuvants for inactivated Newcastle disease vaccine.
Adjuvants, Immunologic
/ administration & dosage
Animals
Chickens
Chitosan
/ administration & dosage
Cytokines
/ immunology
Immunity, Humoral
Lymphocytes
/ drug effects
Mice
Nanoparticles
/ administration & dosage
Newcastle Disease
/ prevention & control
Newcastle disease virus
/ immunology
Poultry Diseases
/ prevention & control
Quaternary Ammonium Compounds
/ administration & dosage
RAW 264.7 Cells
Vaccination
/ methods
Vaccines, Inactivated
/ administration & dosage
Viral Vaccines
/ administration & dosage
Adjuvant
Chitosan
Hydroxypropyltrimethyl ammonium chloride chitosan
Nanoparticle
Newcastle disease virus
Sulfated chitosan
Journal
Carbohydrate polymers
ISSN: 1879-1344
Titre abrégé: Carbohydr Polym
Pays: England
ID NLM: 8307156
Informations de publication
Date de publication:
01 Feb 2020
01 Feb 2020
Historique:
received:
05
03
2019
revised:
19
08
2019
accepted:
01
10
2019
entrez:
13
12
2019
pubmed:
13
12
2019
medline:
21
4
2020
Statut:
ppublish
Résumé
Chitosan (CS) and its water-soluble derivatives, hydroxypropyltrimethyl ammonium chloride chitosan (HACC) and sulfated chitosan (SCS), were used as adjuvants of inactivated Newcastle disease (ND) vaccine. First, NDV-loaded and blank CS, HACC/CS and SCS nanoparticles were prepared. The particle sizes were respectively 343.43 ± 4.12, 320.03 ± 0.84, 156.2 ± 9.29 nm and the zeta potentials were respectively +19.67 ± 0.58, +18.3 ± 0.5, -17.8 ± 2.65 mV under the optimal conditions. Then chickens were immunized with nanoparticles or commercial inactivated oil emulsion vaccine. After immunization, the humoral immunity levels of the chickens were evaluated. The cellular immunity levels were determined by the quantification of cytokines, lymphocyte proliferation assay, the percentages of CD4
Identifiants
pubmed: 31826462
pii: S0144-8617(19)31090-2
doi: 10.1016/j.carbpol.2019.115423
pii:
doi:
Substances chimiques
Adjuvants, Immunologic
0
Cytokines
0
Quaternary Ammonium Compounds
0
Vaccines, Inactivated
0
Viral Vaccines
0
chitosan sulfate
0
hydroxypropyltrimethyl ammonium chloride chitosan
0
Chitosan
9012-76-4
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
115423Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.