G3BP1 inhibits RNA virus replication by positively regulating RIG-I-mediated cellular antiviral response.
DEAD Box Protein 58
/ genetics
DNA Helicases
/ genetics
Gene Expression Regulation
/ immunology
Host-Pathogen Interactions
/ genetics
Humans
Immunity, Innate
/ genetics
Poly-ADP-Ribose Binding Proteins
/ genetics
RNA Helicases
/ genetics
RNA Recognition Motif Proteins
/ genetics
RNA Virus Infections
/ genetics
RNA Viruses
/ genetics
Receptors, Immunologic
Receptors, Pattern Recognition
/ genetics
Signal Transduction
Ubiquitin-Protein Ligases
/ genetics
Ubiquitination
/ genetics
Virus Replication
/ genetics
Journal
Cell death & disease
ISSN: 2041-4889
Titre abrégé: Cell Death Dis
Pays: England
ID NLM: 101524092
Informations de publication
Date de publication:
11 12 2019
11 12 2019
Historique:
received:
29
07
2019
accepted:
21
11
2019
revised:
20
11
2019
entrez:
13
12
2019
pubmed:
13
12
2019
medline:
12
9
2020
Statut:
epublish
Résumé
Retinoic acid-inducible gene I (RIG-I) is a pattern recognition receptor and is involved in the innate immune response against RNA viruses infection. Here, we demonstrate that the Ras-GTPase-activating protein SH3-domain-binding protein 1 (G3BP1) serves as a positive regulator of the RIG-I-mediated signaling pathway. G3BP1-deficient cells inhibited RNA virus-triggered induction of downstream antiviral genes. Furthermore, we found that G3BP1 inhibited the replication of Sendai virus and vesicular stomatitis virus, indicating a positive regulation of G3BP1 to cellular antiviral responses. Mechanistically, G3BP1 formed a complex with RNF125 and RIG-I, leading to decreased RNF125 via its auto-ubiquitination; thus, promoting expression of RIG-I. Overall, the results suggest a novel mechanism for G3BP1 in the positive regulation of antiviral signaling mediated by RIG-I.
Identifiants
pubmed: 31827077
doi: 10.1038/s41419-019-2178-9
pii: 10.1038/s41419-019-2178-9
pmc: PMC6906297
doi:
Substances chimiques
Poly-ADP-Ribose Binding Proteins
0
RNA Recognition Motif Proteins
0
Receptors, Immunologic
0
Receptors, Pattern Recognition
0
RNF125 protein, human
EC 2.3.2.27
Ubiquitin-Protein Ligases
EC 2.3.2.27
RIGI protein, human
EC 3.6.1.-
DNA Helicases
EC 3.6.4.-
G3BP1 protein, human
EC 3.6.4.12
DEAD Box Protein 58
EC 3.6.4.13
RNA Helicases
EC 3.6.4.13
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
946Subventions
Organisme : Gansu Science and Technology Department (Science and Technology Department of Gansu Province)
ID : 17JR5RA323
Pays : International
Organisme : National Natural Science Foundation of China (National Science Foundation of China)
ID : U1501213
Pays : International
Organisme : National Natural Science Foundation of China (National Science Foundation of China)
ID : 31672585
Pays : International
Organisme : Chinese Ministry of Science and Technology | Department of S and T for Social Development (Department of S&T for Social Development)
ID : U1501213
Pays : International
Organisme : Chinese Academy of Agricultural Sciences (CAAS)
ID : Y2017JC55
Pays : International
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