Multiphoton NAD(P)H FLIM reveals metabolic changes in individual cell types of the intact cochlea upon sensorineural hearing loss.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
11 12 2019
11 12 2019
Historique:
received:
01
07
2019
accepted:
27
11
2019
entrez:
13
12
2019
pubmed:
13
12
2019
medline:
11
11
2020
Statut:
epublish
Résumé
An increasing volume of data suggests that changes in cellular metabolism have a major impact on the health of tissues and organs, including in the auditory system where metabolic alterations are implicated in both age-related and noise-induced hearing loss. However, the difficulty of access and the complex cyto-architecture of the organ of Corti has made interrogating the individual metabolic states of the diverse cell types present a major challenge. Multiphoton fluorescence lifetime imaging microscopy (FLIM) allows label-free measurements of the biochemical status of the intrinsically fluorescent metabolic cofactors NADH and NADPH with subcellular spatial resolution. However, the interpretation of NAD(P)H FLIM measurements in terms of the metabolic state of the sample are not completely understood. We have used this technique to explore changes in metabolism associated with hearing onset and with acquired (age-related and noise-induced) hearing loss. We show that these conditions are associated with altered NAD(P)H fluorescence lifetimes, use a simple cell model to confirm an inverse relationship between τ
Identifiants
pubmed: 31827194
doi: 10.1038/s41598-019-55329-x
pii: 10.1038/s41598-019-55329-x
pmc: PMC6906381
doi:
Substances chimiques
Reactive Oxygen Species
0
NADP
53-59-8
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
18907Subventions
Organisme : RCUK | Biotechnology and Biological Sciences Research Council (BBSRC)
ID : BB/L020874/1
Pays : International
Organisme : Action on Hearing Loss
ID : G52
Pays : United Kingdom
Organisme : Action on Hearing Loss
ID : PA12
Pays : United Kingdom
Organisme : Action on Hearing Loss
ID : F66
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/P018726/1
Pays : United Kingdom
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