Depletion of microbiome-derived molecules in the host using

Animals CRISPR-Associated Protein 9 CRISPR-Cas Systems Clostridium / genetics Gastrointestinal Microbiome / genetics Gene Deletion Gene Editing / methods Host Microbial Interactions Metabolic Networks and Pathways / genetics Mice Mice, Inbred Strains

Journal

Science (New York, N.Y.)
ISSN: 1095-9203
Titre abrégé: Science
Pays: United States
ID NLM: 0404511

Informations de publication

Date de publication:
13 12 2019
Historique:
received: 16 08 2018
revised: 14 08 2019
accepted: 23 10 2019
entrez: 14 12 2019
pubmed: 14 12 2019
medline: 31 3 2020
Statut: ppublish

Résumé

The gut microbiota produce hundreds of molecules that are present at high concentrations in the host circulation. Unraveling the contribution of each molecule to host biology remains difficult. We developed a system for constructing clean deletions in

Identifiants

DOI: 10.1126/science.aav1282 PMID: 31831639 PMC: PMC7141153
pubmed: 31831639
pii: 366/6471/eaav1282
doi: 10.1126/science.aav1282
pmc: PMC7141153
mid: NIHMS1559377
pii:
doi:

Substances chimiques

CRISPR-Associated Protein 9 EC 3.1.-

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : NIDDK NIH HHS
ID : R01 DK101674
Pays : United States
Organisme : NIDDK NIH HHS
ID : K08 DK110335
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK085025
Pays : United States
Organisme : NICHD NIH HHS
ID : DP2 HD101401
Pays : United States
Organisme : NIH HHS
ID : DP5 OD023056
Pays : United States
Organisme : NIDDK NIH HHS
ID : DP1 DK113598
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM008568
Pays : United States
Organisme : NIH HHS
ID : S10 OD018040
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK110174
Pays : United States

Commentaires et corrections

Type : CommentIn

Informations de copyright

Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.

Références

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Auteurs

Chun-Jun Guo (CJ)

Department of Bioengineering and ChEM-H, Stanford University, Stanford, CA 94305, USA.
Jill Roberts Institute for Research in Inflammatory Bowel Disease, Department of Medicine, Weill Cornell Medicine, NY 10021, USA.
ORCID: 0000-0002-7227-4591

Breanna M Allen (BM)

Graduate Program in Biomedical Sciences, Departments of Otolaryngology and Microbiology and Immunology, Helen Diller Family Comprehensive Cancer Center, Parker Institute for Cancer Immunotherapy, University of California, San Francisco, San Francisco, CA 94143, USA.
Chan Zuckerberg Biohub, San Francisco, CA 94158, USA.
ORCID: 0000-0002-4830-6792

Kamir J Hiam (KJ)

Graduate Program in Biomedical Sciences, Departments of Otolaryngology and Microbiology and Immunology, Helen Diller Family Comprehensive Cancer Center, Parker Institute for Cancer Immunotherapy, University of California, San Francisco, San Francisco, CA 94143, USA.
Chan Zuckerberg Biohub, San Francisco, CA 94158, USA.
ORCID: 0000-0002-1956-0257

Dylan Dodd (D)

Department of Pathology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA.

Will Van Treuren (W)

Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Chan Zuckerberg Biohub, San Francisco, CA 94158, USA.

Steven Higginbottom (S)

Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Chan Zuckerberg Biohub, San Francisco, CA 94158, USA.
ORCID: 0000-0002-4609-6840

Kazuki Nagashima (K)

Department of Bioengineering and ChEM-H, Stanford University, Stanford, CA 94305, USA.
ORCID: 0000-0002-8593-136X

Curt R Fischer (CR)

Department of Bioengineering and ChEM-H, Stanford University, Stanford, CA 94305, USA.
Chan Zuckerberg Biohub, San Francisco, CA 94158, USA.
ORCID: 0000-0003-3386-9813

Justin L Sonnenburg (JL)

Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Chan Zuckerberg Biohub, San Francisco, CA 94158, USA.
ORCID: 0000-0003-2299-6817

Matthew H Spitzer (MH)

Graduate Program in Biomedical Sciences, Departments of Otolaryngology and Microbiology and Immunology, Helen Diller Family Comprehensive Cancer Center, Parker Institute for Cancer Immunotherapy, University of California, San Francisco, San Francisco, CA 94143, USA. fischbach@fischbachgroup.org matthew.spitzer@ucsf.edu.
Chan Zuckerberg Biohub, San Francisco, CA 94158, USA.
ORCID: 0000-0002-5291-3819

Michael A Fischbach (MA)

Department of Bioengineering and ChEM-H, Stanford University, Stanford, CA 94305, USA. fischbach@fischbachgroup.org matthew.spitzer@ucsf.edu.
Chan Zuckerberg Biohub, San Francisco, CA 94158, USA.
ORCID: 0000-0003-3079-8247

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Depletion of microbiome-derived molecules in...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines associées aux CRISPR › Protéine-9 associée à CRISPR › Depletion of microbiome-derived molecules in...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Épigenèse génétique › Extinction de l'expression des gènes › Systèmes CRISPR-Cas › Depletion of microbiome-derived molecules in...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bactéries à Gram positif formant des endospores › Clostridium › Depletion of microbiome-derived molecules in...
questionsmedicales.fr › Environnement et santé publique › Environnement › Écosystème › Biodiversité › Biote › Microbiote › Microbiome gastro-intestinal › Depletion of microbiome-derived molecules in...
questionsmedicales.fr › Phénomènes génétiques › Mutagenèse › Délétion de séquence › Délétion de gène › Depletion of microbiome-derived molecules in...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Génie génétique › Édition de gène › Depletion of microbiome-derived molecules in...
questionsmedicales.fr › Phénomènes biologiques › Interactions hôte-microbes › Depletion of microbiome-derived molecules in...
questionsmedicales.fr › Métabolisme › Voies et réseaux métaboliques › Depletion of microbiome-derived molecules in...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Depletion of microbiome-derived molecules in...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Lignées consanguines de souris › Depletion of microbiome-derived molecules in...