IL-10 IS DOWN-REGULATED IN THE CARDIOVASCULAR DISEASES SUSPECTED PATIENTS, INDEPENDENT OF ANGIOGRAPHY.

IL-10 and interferon-gamma (IFN-γ) are the important anti and pro-inflammatory cytokines, respectively, which may participate in the cardiovascular disease pathogenesis. Additionally, environmental factors, such as the X-ray, can modulate cytokine expression. Due to the fact that X-ray is used during angiography, hence, angiography may alter expression of the cytokines. Accordingly, this project was aimed to assess IL-10 and IFN-γ serum levels within cardiovascular patients (with and without vessel stenosis) versus healthy controls and also the effects of angiography on the serum levels of the cytokines. This study was performed on the 80 participants, including twenty cases in each group (healthy controls and cardiovascular patients without vessel stenosis, stenosis of 1 vessel and stenosis of more than 1 vessel) to evaluate IL-10 and IFN-γ serum levels using ELISA technique. The IL-10 and IFN-γ serum levels also compared within group 2, 3 and 4 before and after angiography to explore the effects of the technique on the IL-10 and IFN-γ serum levels. IL-10, but not IFN-γ, serum levels were higher in the healthy controls than all cardiovascular patients. IL-10 and IFN-γ serum levels were not altered after angiography and also were not differ in the smoker versus non- smoker and opium consuming versus non-opium consuming participants. Due to the results it may be concluded that IL-10 can be considered as a plausible inhibitor of cardio- vascular diseases independent of angiography duration and X-ray, however, IFN-γ has no effects in the Iranian patients suffering from cardiovascular diseases. Interley̆kin-10 (IL-10) ta interferon-gamma (IFN-γ) ie vazhlyvymy, vidpovidno, proty- ta prozapal'nymy tsy- tokinamy, iaki mozhut' braty uchast' u patogenezi sertsevo-sudynnykh zakhvoriuvan'. Krim togo, faktory navkolysh- n'ogo seredovyshcha – taki, iak rentgenivs'ke vyprominiuvannia – zdatni moduliuvaty ekspresiiu tsytokiniv. Cherez te, shcho rentgenivs'ke vyprominiuvannia vykorystovuiet'sia pry angiografiï, tsia protsedura, mozhlyvo, zminiuie ekspresiiu tsytokiniv.Meta. Otsinyty rivni IL-10 ta IFN-γ syrovatky krovi u patsiientiv iz sertsevo-sudynnymy zakhvoriuvanniamy (u iakykh naiavnyy̆ abo zh vidsutniy̆ stenoz sudyn) porivniano zi zdorovymy kontroliamy, a takozh efekty angiografiï shchodo vmistu tsytokiniv u syrovattsi krovi.Materialy ta metody. Doslidzhennia bulo provedeno sered 80 uchasnykiv – po 20 vypadkiv u kozhniy̆ grupi (zdo- rovi kontroli ta osoby iz sertsevo-sudynnymy zakhvoriuvanniamy bez stenozu sudyn, iz stenozom 1 sudyny ta ste- nozom bil'sh nizh 1 sudyny) dlia otsinky rivniv IL-10 ta IFN-γ u syrovattsi krovi za dopomogoiu metodu imunofer- mentnogo analizu. Dlia z’iasuvannia vplyvu protsedury angiografiï na riven' IL-10 ta IFN-γ u syrovattsi krovi, vmist obokh tsytokiniv takozh porivniuvaly u grupakh 2, 3 ta 4 do ta pislia angiografichnogo doslidzhennia. Rezul'taty. Riven' IL-10, ale ne IFN-γ, u syrovattsi krovi zdorovykh osib grupy kontroliu buv bil'shym, nizh u vsikh patsiientiv iz sertsevo-sudynnymy zakhvoriuvanniamy. Riven' IL-10 ta IFN-γ u syrovattsi krovi ne zminiuvavsia pislia angiografiï, a takozh ne vidrizniavsia u kurtsiv ta osib, iaki ne kuriat', tak samo iak i u spozhyvachiv opiiu ta osib, iaki opiy̆ ne vzhyvaiut'.Vysnovky. Zavdiaky rezul'tatam mozhna zrobyty vysnovok, shcho IL-10 mozhna y̆movirno vvazhaty ingibitorom ser- tsevo-sudynnykh zakhvoriuvan', nezalezhno vid tryvalosti angiografiï ta rentgenografiï, v toy̆ chas iak IFN-γ ne pro- iavliaie efektiv u irans'kykh patsiientiv, iaki strazhdaiut' na sertsevo-sudynni zakhvoriuvannia.

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S. Razavi, F. Ahmadi-Roknabadi, M. Safarian, A. Mehdipour, A. Anbarian, M. Mirzamohammadi, M. Zeinali, M. Kazemi Arababadi.