Ten-Second Electrophysiology: Evaluation of the 3DEP Platform for high-speed, high-accuracy cell analysis.


Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
16 12 2019
Historique:
received: 22 08 2019
accepted: 18 11 2019
entrez: 18 12 2019
pubmed: 18 12 2019
medline: 6 11 2020
Statut: epublish

Résumé

Electrical correlates of the physiological state of a cell, such as membrane conductance and capacitance, as well as cytoplasm conductivity, contain vital information about cellular function, ion transport across the membrane, and propagation of electrical signals. They are, however, difficult to measure; gold-standard techniques are typically unable to measure more than a few cells per day, making widespread adoption difficult and limiting statistical reproducibility. We have developed a dielectrophoretic platform using a disposable 3D electrode geometry that accurately (r

Identifiants

pubmed: 31844107
doi: 10.1038/s41598-019-55579-9
pii: 10.1038/s41598-019-55579-9
pmc: PMC6915758
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

19153

Subventions

Organisme : British Heart Foundation
ID : FS/17/33/32931
Pays : United Kingdom

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Auteurs

Kai F Hoettges (KF)

Centre for Biomedical Engineering, Department of Mechanical Engineering Sciences, University of Surrey, Guildford, Surrey, GU2 7XH, UK.
Department of Electrical Engineering and Electronics, University of Liverpool, Brownlow Hill, Liverpool, L69 3GJ, UK.

Erin A Henslee (EA)

Centre for Biomedical Engineering, Department of Mechanical Engineering Sciences, University of Surrey, Guildford, Surrey, GU2 7XH, UK.
Department of Engineering, Wake Forest University, Wake Downtown, Winston-Salem, NC 27109, USA.

Ruth M Torcal Serrano (RM)

Centre for Biomedical Engineering, Department of Mechanical Engineering Sciences, University of Surrey, Guildford, Surrey, GU2 7XH, UK.

Rita I Jabr (RI)

School of Biosciences and Medicine, University of Surrey, Guildford, Surrey, GU2 7XH, UK.

Rula G Abdallat (RG)

Department of Biomedical Engineering, Faculty of Engineering, The Hashemite University, PO Box 330127, Zarqa, 13133, Jordan.

Andrew D Beale (AD)

Centre for Biomedical Engineering, Department of Mechanical Engineering Sciences, University of Surrey, Guildford, Surrey, GU2 7XH, UK.
MRC Laboratory for Molecular Biology, Francis Crick Avenue, Cambridge, CB2 0QH, UK.

Abdul Waheed (A)

School of Biosciences and Medicine, University of Surrey, Guildford, Surrey, GU2 7XH, UK.

Patrizia Camelliti (P)

School of Biosciences and Medicine, University of Surrey, Guildford, Surrey, GU2 7XH, UK.

Christopher H Fry (CH)

School of Biosciences and Medicine, University of Surrey, Guildford, Surrey, GU2 7XH, UK.
School of Physiology, Pharmacology & Neuroscience, University of Bristol, University Walk, Bristol, BS8 1TD, UK.

Daan R van der Veen (DR)

School of Biosciences and Medicine, University of Surrey, Guildford, Surrey, GU2 7XH, UK.

Fatima H Labeed (FH)

Centre for Biomedical Engineering, Department of Mechanical Engineering Sciences, University of Surrey, Guildford, Surrey, GU2 7XH, UK. f.labeed@surrey.ac.uk.

Michael P Hughes (MP)

Centre for Biomedical Engineering, Department of Mechanical Engineering Sciences, University of Surrey, Guildford, Surrey, GU2 7XH, UK. m.hughes@surrey.ac.uk.

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