GC content shapes mRNA storage and decay in human cells.
GC content
P-bodies
chromosomes
codon usage
gene expression
human
mRNA decay
mRNA storage
post-transcriptional regulation
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
19 12 2019
19 12 2019
Historique:
received:
26
06
2019
accepted:
18
12
2019
pubmed:
20
12
2019
medline:
23
5
2020
entrez:
20
12
2019
Statut:
epublish
Résumé
mRNA translation and decay appear often intimately linked although the rules of this interplay are poorly understood. In this study, we combined our recent P-body transcriptome with transcriptomes obtained following silencing of broadly acting mRNA decay and repression factors, and with available CLIP and related data. This revealed the central role of GC content in mRNA fate, in terms of P-body localization, mRNA translation and mRNA stability: P-bodies contain mostly AU-rich mRNAs, which have a particular codon usage associated with a low protein yield; AU-rich and GC-rich transcripts tend to follow distinct decay pathways; and the targets of sequence-specific RBPs and miRNAs are also biased in terms of GC content. Altogether, these results suggest an integrated view of post-transcriptional control in human cells where most translation regulation is dedicated to inefficiently translated AU-rich mRNAs, whereas control at the level of 5' decay applies to optimally translated GC-rich mRNAs.
Identifiants
pubmed: 31855182
doi: 10.7554/eLife.49708
pii: 49708
pmc: PMC6944446
doi:
pii:
Substances chimiques
MicroRNAs
0
RNA, Messenger
0
RNA, Messenger, Stored
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Association pour la Recherche sur le Cancer
ID : Subvention Fixe
Pays : International
Organisme : Agence Nationale de la Recherche
ID : ANR-14-CE09-0013-01
Pays : International
Organisme : European Research Council
ID : DARK consolidator grant
Pays : International
Organisme : Agence Nationale de la Recherche
ID : ANR-11-LABX-0028-01
Pays : International
Informations de copyright
© 2019, Courel et al.
Déclaration de conflit d'intérêts
MC, YC, CB, DF, OV, ZY, MB, MB, MK, CV, ME, CA, AM, PB, AH, HR, NS, DW No competing interests declared
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