Development of Mammalian Cell Perfusion Cultures at Lab Scale: From Orbitally Shaken Tubes to Benchtop Bioreactors.

Animals Bioreactors CHO Cells Cell Count Cell Culture Techniques / instrumentation Cell Survival / physiology Cricetulus Culture Media / chemistry Perfusion / methods
Benchtop bioreactors CHO cells Perfusion process development Scale-down models Shake tubes

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2020
Historique:
entrez: 21 12 2019
pubmed: 21 12 2019
medline: 3 2 2021
Statut: ppublish

Résumé

This chapter introduces the necessary concepts to develop mammalian cell perfusion cultures for the expression of therapeutic proteins at lab scale. We highlight the operation of the orbitally shaken tubes and of a classical glass vessel reactor system coupled to an external alternating tangential flow (ATF) device. Two different experiments can be performed in the shake-tube system: (1) the VCD

Identifiants

DOI: 10.1007/978-1-0716-0191-4_8 PMID: 31858466
pubmed: 31858466
doi: 10.1007/978-1-0716-0191-4_8
doi:

Substances chimiques

Culture Media 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

125-140

Auteurs

Moritz Wolf (M)

Institute for Chemical and Bioengineering, ETH Zurich, Zurich, Switzerland. moritz.wolf@chem.ethz.ch.

Massimo Morbidelli (M)

Institute for Chemical and Bioengineering, ETH Zurich, Zurich, Switzerland.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Development of Mammalian Cell Perfusion...
questionsmedicales.fr › Technologie, industrie et agriculture › Technologie › Biotechnologie › Bioréacteurs › Development of Mammalian Cell Perfusion...
questionsmedicales.fr › Cellules › Cellules épithéliales › Cellules CHO › Development of Mammalian Cell Perfusion...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Numération cellulaire › Development of Mammalian Cell Perfusion...
questionsmedicales.fr › Techniques d'investigation › Techniques in vitro › Techniques de culture › Techniques de culture cellulaire › Development of Mammalian Cell Perfusion...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Survie cellulaire › Development of Mammalian Cell Perfusion...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Arvicolinae › Cricetinae › Cricetulus › Development of Mammalian Cell Perfusion...
questionsmedicales.fr › Équipement et fournitures › Milieux de culture › Development of Mammalian Cell Perfusion...
questionsmedicales.fr › Techniques d'investigation › Perfusion › Development of Mammalian Cell Perfusion...