The Deoptimization of Rabies Virus Matrix Protein Impacts Viral Transcription and Replication.
codon deoptimization
glycoprotein
matrix protein
rabies virus
replication
Journal
Viruses
ISSN: 1999-4915
Titre abrégé: Viruses
Pays: Switzerland
ID NLM: 101509722
Informations de publication
Date de publication:
18 12 2019
18 12 2019
Historique:
received:
19
11
2019
revised:
06
12
2019
accepted:
16
12
2019
entrez:
22
12
2019
pubmed:
22
12
2019
medline:
29
9
2020
Statut:
epublish
Résumé
Rabies virus (RABV) matrix (M) protein plays several important roles during RABV infection. Although previous studies have assessed the functions of M through gene rearrangements, this interferes with the position of other viral proteins. In this study, we attenuated M expression through deoptimizing its codon usage based on codon pair bias in RABV. This strategy more objectively clarifies the role of M during virus infection. Codon-deoptimized M inhibited RABV replication during the early stages of infection, but enhanced viral titers at later stages. Codon-deoptimized M also inhibited genome synthesis at early stage of infection and increased the RABV transcription rates. Attenuated M through codon deoptimization enhanced RABV glycoprotein expression following RABV infection in neuronal cells, but had no influence on the cell-to-cell spread of RABV. In addition, codon-deoptimized M virus induced higher levels of apoptosis compared to the parental RABV. These results indicate that codon-deoptimized M increases glycoprotein expression, providing a foundation for further investigation of the role of M during RABV infection.
Identifiants
pubmed: 31861477
pii: v12010004
doi: 10.3390/v12010004
pmc: PMC7019236
pii:
doi:
Substances chimiques
Codon
0
Viral Matrix Proteins
0
Viral Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Références
J Virol. 2002 Apr;76(7):3374-81
pubmed: 11884563
J Virol. 2006 Apr;80(8):3701-11
pubmed: 16571787
DNA Res. 2014 Oct;21(5):511-26
pubmed: 24906480
mBio. 2015 Mar 31;6(2):
pubmed: 25827413
PLoS One. 2014 Feb 03;9(2):e87105
pubmed: 24498294
J Virol. 2011 Jan;85(2):697-704
pubmed: 21068252
Front Cell Infect Microbiol. 2017 Apr 26;7:146
pubmed: 28491824
Arch Virol. 2018 Jul;163(7):1889-1895
pubmed: 29594364
J Gen Virol. 2003 Jun;84(Pt 6):1613-1621
pubmed: 12771432
Science. 2008 Jun 27;320(5884):1784-7
pubmed: 18583614
Microbiol Immunol. 2010 Feb;54(2):89-97
pubmed: 20377742
Virology. 2014 Nov;468-470:621-630
pubmed: 25310498
J Virol. 2003 Oct;77(19):10537-47
pubmed: 12970438
Virus Genes. 2017 Aug;53(4):573-583
pubmed: 28447192
Nat Rev Mol Cell Biol. 2018 Jan;19(1):20-30
pubmed: 29018283
Arch Virol. 2016 Oct;161(10):2863-70
pubmed: 27438075
Adv Virus Res. 2011;79:1-22
pubmed: 21601039
J Mol Evol. 2008 Mar;66(3):210-23
pubmed: 18286220
PLoS Pathog. 2012;8(12):e1003060
pubmed: 23271966
Virus Genes. 2016 Jun;52(3):379-87
pubmed: 26957093
PLoS Pathog. 2017 Oct 30;13(10):e1006697
pubmed: 29084252
J Virol. 2005 Nov;79(22):14141-8
pubmed: 16254349
Nucleic Acids Res. 2007;35(5):1522-32
pubmed: 17287294
J Virol. 1999 Jan;73(1):242-50
pubmed: 9847327
Sci Rep. 2016 Dec 21;6:39420
pubmed: 28000711
Virusdisease. 2014;25(3):365-71
pubmed: 25674605
J Virol. 2006 Oct;80(19):9687-96
pubmed: 16973573
J Virol. 1999 Jan;73(1):510-8
pubmed: 9847357
J Biol Chem. 2005 Apr 8;280(14):13512-9
pubmed: 15705563
Virus Res. 2005 Aug;111(2):120-31
pubmed: 15885837
J Virol. 1994 Jan;68(1):555-60
pubmed: 8254771
Nucleic Acids Res. 1986 Jul 11;14(13):5125-43
pubmed: 3526280
Front Microbiol. 2017 Feb 03;8:120
pubmed: 28217116
Cell. 1996 Mar 22;84(6):941-51
pubmed: 8601317
J Virol. 2003 Nov;77(22):12074-82
pubmed: 14581544
Virus Res. 2004 Dec;106(2):117-32
pubmed: 15567492
Microbiol Immunol. 1999;43(3):259-70
pubmed: 10338196