Modeling the nonlinear behavior of a bioactive peptide in reversed-phase gradient elution chromatography.
Adsorption isotherms
Gradient elution
Inverse method
Pharmaceutical peptides
Preparative chromatography
Reversed-phase liquid chromatography (RP-LC)
Journal
Journal of chromatography. A
ISSN: 1873-3778
Titre abrégé: J Chromatogr A
Pays: Netherlands
ID NLM: 9318488
Informations de publication
Date de publication:
12 Apr 2020
12 Apr 2020
Historique:
received:
22
10
2019
revised:
09
12
2019
accepted:
12
12
2019
pubmed:
26
12
2019
medline:
18
6
2020
entrez:
26
12
2019
Statut:
ppublish
Résumé
The thermodynamic behavior of octreotide, a cyclic octapeptide with important pharmaceutical functions, has been simulated under reversed-phase gradient elution conditions. To this end, adsorption behavior was firstly investigated in isocratic conditions, under a variety of water/acetonitrile + 0.02% (v/v) trifluoroacetic acid (TFA) mixtures as mobile phase by using a Langmuir isotherm. Organic modifier was varied in the range between 23 and 28% (v/v). Adsorption isotherms were determined by means of the so-called Inverse Method (IM) with a minimum amount of peptide. The linear solvent strength (LSS) model was used to find the correlation between isotherm parameters and mobile phase composition. This study contributes to enlarge our knowledge on the chromatographic behavior under nonlinear gradient conditions of peptides. In particular, it focuses on a cyclic octapeptide.
Identifiants
pubmed: 31874699
pii: S0021-9673(19)31237-3
doi: 10.1016/j.chroma.2019.460789
pii:
doi:
Substances chimiques
Peptides
0
Solvents
0
Water
059QF0KO0R
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
460789Informations de copyright
Copyright © 2019. Published by Elsevier B.V.
Déclaration de conflit d'intérêts
Declaration of Competing Interest None.