Polyhexamethylene Guanidine Phosphate Damages Tight Junctions and the F-Actin Architecture by Activating Calpain-1 via the P2RX7/Ca
Actins
/ metabolism
Antigens, CD
/ metabolism
Apyrase
/ metabolism
Cadherins
/ metabolism
Calcium
/ metabolism
Calpain
/ metabolism
Cell Line
Epithelial Cells
/ drug effects
Guanidines
/ metabolism
Humans
Lung
/ metabolism
Pulmonary Fibrosis
/ metabolism
Receptors, Purinergic P2X7
/ metabolism
Signal Transduction
/ drug effects
Tight Junctions
/ metabolism
F-actin
P2RX7
PHMG-p
calpain
tight junctions
Journal
Cells
ISSN: 2073-4409
Titre abrégé: Cells
Pays: Switzerland
ID NLM: 101600052
Informations de publication
Date de publication:
24 Dec 2019
24 Dec 2019
Historique:
received:
14
11
2019
revised:
20
12
2019
accepted:
22
12
2019
entrez:
28
12
2019
pubmed:
28
12
2019
medline:
2
9
2020
Statut:
epublish
Résumé
Polyhexamethylene guanidine phosphate (PHMG-p), a member of the polymeric guanidine family, has strong antimicrobial activity and may increase the risk of inflammation-associated pulmonary fibrosis. However, the effect of PHMG-p on the barrier function of the bronchial epithelium is unknown. Epithelial barrier functioning is maintained by tight junctions (TJs); damage to these TJs is the major cause of epithelial barrier breakdown during lung inflammation. The present study showed that, in BEAS-2B human bronchial epithelial cells, exposure to PHMG-p reduced the number of TJs and the E-cadherin level and impaired the integrity of the F-actin architecture. Furthermore, exposure to PHMG-p stimulated the calcium-dependent protease calpain-1, which breaks down TJs. However, treatment with the calpain-1 inhibitor, ALLN, reversed the PHMG-p-mediated impairment of TJs and the F-actin architecture. Furthermore, exposure to PHMG-p increased the intracellular Ca
Identifiants
pubmed: 31878359
pii: cells9010059
doi: 10.3390/cells9010059
pmc: PMC7016582
pii:
doi:
Substances chimiques
Actins
0
Antigens, CD
0
CDH1 protein, human
0
Cadherins
0
Guanidines
0
P2RX7 protein, human
0
Receptors, Purinergic P2X7
0
polyhexamethyleneguanidine
31961-54-3
Calpain
EC 3.4.22.-
Apyrase
EC 3.6.1.5
CD39 antigen
EC 3.6.1.5
Calcium
SY7Q814VUP
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Korea Ministry of Environment (MOE) as "the Environmental Health Action Program"
ID : 2017001360001
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
Références
Molecules. 2018 Aug 07;23(8):
pubmed: 30087305
N Engl J Med. 2000 May 4;342(18):1334-49
pubmed: 10793167
Arch Toxicol. 2016 Mar;90(3):617-32
pubmed: 25716161
Eur Respir J. 2012 Feb;39(2):419-28
pubmed: 21778164
Tissue Barriers. 2013 Apr 1;1(2):e24783
pubmed: 24665393
Annu Rev Cell Dev Biol. 2007;23:237-61
pubmed: 17539752
J Immunol. 2009 Apr 1;182(7):4378-85
pubmed: 19299738
Int J Mol Sci. 2013 May 10;14(5):9906-46
pubmed: 23665898
Biochim Biophys Acta. 2008 Mar;1778(3):660-9
pubmed: 17854762
Biochim Biophys Acta. 1984 Jan 11;769(1):57-66
pubmed: 6691980
Neural Plast. 2016;2016:8928530
pubmed: 27795859
Br J Pharmacol. 2007 Feb;150(4):445-54
pubmed: 17211459
Semin Cell Dev Biol. 2015 Jun;42:47-57
pubmed: 25951797
J Med Microbiol. 2008 Dec;57(Pt 12):1523-8
pubmed: 19018024
Biol Pharm Bull. 2001 Sep;24(9):1093-6
pubmed: 11558578
Nat Rev Mol Cell Biol. 2001 Apr;2(4):285-93
pubmed: 11283726
Science. 2014 Nov 21;346(6212):1000-3
pubmed: 25414314
J Appl Bacteriol. 1984 Aug;57(1):115-24
pubmed: 6386785
J Med Microbiol. 2012 Oct;61(Pt 10):1421-7
pubmed: 22871428
Biochim Biophys Acta. 1983 Nov 23;735(3):380-6
pubmed: 6639946
Pflugers Arch. 2017 Jan;469(1):135-147
pubmed: 27921210
J Biomed Sci. 2013 May 01;20:26
pubmed: 23634990
Am J Physiol. 1987 Dec;253(6 Pt 1):C854-61
pubmed: 3425707
J Biol Chem. 2011 Jan 28;286(4):2750-61
pubmed: 21084306
Front Biosci. 2008 May 01;13:6662-81
pubmed: 18508686
Results Immunol. 2014 Aug 01;4:62-7
pubmed: 25379376
J Toxicol Sci. 2016;41(6):711-717
pubmed: 27853099
J Clin Invest. 1986 Dec;78(6):1513-22
pubmed: 2431002
Purinergic Signal. 2015 Dec;11(4):435-40
pubmed: 26318434
Immunology. 1992 Nov;77(3):330-7
pubmed: 1478679
J Immunol. 2011 Jun 1;186(11):6119-28
pubmed: 21508263
Part Fibre Toxicol. 2012 Aug 29;9:35
pubmed: 22931549
Biochim Biophys Acta. 2008 Mar;1778(3):588-600
pubmed: 17916321
Toxicology. 2019 Feb 15;414:35-44
pubmed: 30629986
EMBO J. 2002 Apr 15;21(8):1948-56
pubmed: 11953314
J Immunol. 2004 Apr 15;172(8):4987-94
pubmed: 15067080
Int J Mol Sci. 2019 May 09;20(9):
pubmed: 31075901
Toxicol Lett. 2018 Apr;286:108-119
pubmed: 29309811
Am J Physiol Lung Cell Mol Physiol. 2017 Mar 1;312(3):L309-L325
pubmed: 27979859
J Endocrinol. 2012 May;213(2):123-41
pubmed: 22396456
Science. 1996 May 3;272(5262):735-8
pubmed: 8614837