A genetic toolkit for the analysis of metabolic changes in Drosophila provides new insights into metabolic responses to stress and malignant transformation.
Animals
Cell Line, Tumor
Cell Proliferation
Cell Transformation, Neoplastic
Drosophila melanogaster
/ genetics
Energy Metabolism
/ genetics
Fluorescence Resonance Energy Transfer
/ methods
Glycolysis
/ physiology
Hypoxia
/ metabolism
Image Processing, Computer-Assisted
/ methods
Ketoglutaric Acids
/ analysis
Lactic Acid
/ analysis
Oxygen
/ metabolism
Pyruvic Acid
/ analysis
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
27 12 2019
27 12 2019
Historique:
received:
15
08
2019
accepted:
25
11
2019
entrez:
29
12
2019
pubmed:
29
12
2019
medline:
4
11
2020
Statut:
epublish
Résumé
Regulation of the energetic metabolism occurs fundamentally at the cellular level, so analytical strategies must aim to attain single cell resolution to fully embrace its inherent complexity. We have developed methods to utilize a toolset of metabolic FRET sensors for assessing lactate, pyruvate and 2-oxoglutarate levels of Drosophila tissues in vivo by imaging techniques. We show here how the energetic metabolism is altered by hypoxia: While some larval tissues respond to low oxygen levels by executing a metabolic switch towards lactic fermentation, the fat body and salivary glands do not alter their energetic metabolism. Analysis of tumor metabolism revealed that depending on the genetic background, some tumors undergo a lactogenic switch typical of the Warburg effect, while other tumors do not. This toolset allows for developmental and physiologic studies in genetically manipulated Drosophila individuals in vivo.
Identifiants
pubmed: 31882718
doi: 10.1038/s41598-019-56446-3
pii: 10.1038/s41598-019-56446-3
pmc: PMC6934733
doi:
Substances chimiques
Ketoglutaric Acids
0
alpha-ketoglutaramate
18465-19-5
Lactic Acid
33X04XA5AT
Pyruvic Acid
8558G7RUTR
Oxygen
S88TT14065
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
19945Références
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