Carboxymethyl cellulose/diclofenac bioactive coatings on AISI 316LVM for controlled drug delivery, and improved osteogenic potential.
Anti-Inflammatory Agents, Non-Steroidal
/ administration & dosage
Biocompatible Materials
/ chemistry
Bone Morphogenetic Protein 2
/ genetics
Carboxymethylcellulose Sodium
/ chemistry
Cell Proliferation
Cells, Cultured
Diclofenac
/ administration & dosage
Drug Liberation
Extracellular Matrix
/ metabolism
Extracellular Matrix Proteins
/ genetics
Humans
Osteoblasts
/ drug effects
Osteogenesis
Proteoglycans
/ genetics
Steel
/ chemistry
Biocompatibility
Carboxymethyl cellulose
Controlled-drug release
Diclofenac
Gene expression
Journal
Carbohydrate polymers
ISSN: 1879-1344
Titre abrégé: Carbohydr Polym
Pays: England
ID NLM: 8307156
Informations de publication
Date de publication:
15 Feb 2020
15 Feb 2020
Historique:
received:
29
08
2019
revised:
11
10
2019
accepted:
12
11
2019
entrez:
1
1
2020
pubmed:
1
1
2020
medline:
5
6
2020
Statut:
ppublish
Résumé
This work reports on the preparation and systematic testing of a novel multi-layered coating, comprised of the non-steroid anti-inflammatory drug diclofenac and biopolymer carboxymethyl cellulose. Drug release testing was performed on an Automated Transdermal Diffusion Cells Sampling System in combination with UV-VIS spectroscopy as the released drug concentration determination method. The results showed that most of the drug is released in the first six hours, whereas the overall released amount could be tailored through changes in the multi-layered coating composition. Biocompatibility tests performed on human osteoblast cells, showed cell viability improvement between 7% and 17% compared to the control sample. The expression of proteins playing important roles in extracellular matrix production and functioning was performed in order to obtain additional proof of the prepared materials' osteointegration boosting capacity. Finally, electrochemical measurements confirmed that the coatings do not influence the corrosion susceptibility of AISI 316LVM stainless steel.
Identifiants
pubmed: 31887952
pii: S0144-8617(19)31280-9
doi: 10.1016/j.carbpol.2019.115612
pii:
doi:
Substances chimiques
Anti-Inflammatory Agents, Non-Steroidal
0
Biocompatible Materials
0
Bone Morphogenetic Protein 2
0
Extracellular Matrix Proteins
0
Proteoglycans
0
osteoadherin
0
Steel
12597-69-2
Diclofenac
144O8QL0L1
Carboxymethylcellulose Sodium
K679OBS311
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
115612Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.